The 407.6G12 monoclonal antibody reacts with phosphorylated mouse PD-1. Specifically, the antibody only reacts with PD-1 when tyrosine 248 (Y248) is phosphorylated. PD-1 is a 50-55 kDa cell surface receptor that belongs to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 ａnd CD8 thymocytes as well as activated T ａnd B lymphocytes ａnd myeloid cells. PD-1 expression declines after successful elimination of antigen. PD-1 signals via binding its two ligands, PD-L1 ａnd PD-L2. Upon ligand binding, PD-1 signaling inhibits T-cell activation, leading to reduced proliferation, cytokine production, ａnd T-cell death. Phosphorylation of Y248 of the ITSM motif of PD-1 is essential for the initiation of inhibitory signals. PD-1 blockade leads to reduced phosphor-PD-1. The 6G12 antibody can be used to assess the efficacy of PD-1 pathway blockade.

BioXCell体内MAb小鼠/人磷酸化PD-1(CD279)抗体：

407.6G12单克隆抗体与磷酸化小鼠PD-1反应。具体来说，抗体仅在酪氨酸1（Y248）磷酸化时与PD-248反应。PD-1是一种50-55 kDa的细胞表面受体，属于Ig超家族的CD28家族。PD-1在CD4和CD8胸腺细胞以及活化的T和B淋巴细胞和骨髓细胞上短暂表达。PD-1在成功消除抗原后表达下降。

BioXCell艾美捷体内MAb小鼠/人磷酸化PD-1(CD279)抗体规格：

#  BXC-BE0387

Isotype Mouse IgG2a, κ

Recommended Isotype Control(s) InVivoMAb mouse IgG2a isotype control, unknown specificity

Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer

Immunogen Human PD-1 p248Tyr peptide conjugated to KLH

Reported Applications Western Blot

Flow Cytometry

Formulation PBS, pH 7.0

Contains no stabilizers or preservatives

Endotoxin <2EU/mg (<0.002EU/μg)

Determined by LAL gel clotting assay

Purity >95%

Determined by SDS-PAGE

Sterility 0.2 µm filtration

Production Purified from tissue culture supernatant in an animal free facility

Purification Protein G

Molecular Weight 150 kDa

Storage The antibody solution should be stored at the stock concentration at 4°C.

同种型 小鼠IgG2a， κ

推荐的同种型对照 体内MAb小鼠IgG2a同种型对照，特异性未知

推荐的稀释缓冲液 体内纯 pH 7.0 稀释缓冲液

免疫原 与KLH偶联的人PD-1 p248Tyr肽

报告的应用程序 蛋白质印迹

流式细胞术

配方 PBS，pH 7.0

不含稳定剂或防腐剂

内毒素 <2EU/mg （<0.002EU/μg）

通过LAL凝胶凝血测定法测定

纯度 >95%

由 SDS-PAGE 决定

无 菌 0.2 μm 过滤

生产 从无动物设施中的组织培养上清液中纯化

纯化 蛋白 G

分子量 150 千达

存储 抗体溶液应以4°C的储备浓度储存。 不要冷冻。

来源：https://www.amyjet.com/products/BXC-BE0387-1mg.shtml