Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, ａnd an aminopeptidase. This combination of collagenolytic ａnd proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Crude collagenase is inhibited by metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by α2-macroglobulin, a large plasma glycoprotein. Ca2+ is required for enzyme activity. Particular enzymatic profiles of each collagenase have been correlated with the tissues from which the cells for study were obtained (or with the uses to which the cells are put) ａnd as a result of the correlations several types of crude collagenases have been established by Worthington: Types 1, 2, 3, ａnd 4.

• Type 1 crude collagenase has the original balance of collagenase, caseinase, clostripain ａnd tryptic activities.

• Type 2 contains higher relative levels of protease activity, particularly clostripain.

• Type 3 contains lowest levels of secondary proteases.

• Type 4 is designed to be especially low in tryptic activity to limit damage to membrane proteins ａnd receptors.

• Type 5 contains higher collagenase ａnd caseinase values.

• Type 6 contains high collagenase activity with a caseinase to collagenase ratio ~2:1. Designed to be enriched for Type II (col H) collagenase relative to Type I (col G).

• Type 7 contains collagenase ａnd caseinase activities four-fold higher than collagenase Types 1 ａnd 2.

Related Products:

Collagen (CL)

Deoxyribonuclease I (DP/D/DCLS/D2/DPFF/DPRF)

Elastase (ES/ESL/ESFF)

Hyaluronidase (HSE/HSEP)

Neutral Protease (Dispase, NPRO)

Papain (PAP/PAPL/PAP2)Proteinase K (PROK)

Trypsin (TL/TRL/TRL3/TRLS/TRTPCK)

Trypsin Inhibitors (LBI/OI/SI/SIC)

Cell Isolation Optimizing System (CIT)

Hepatocyte Isolation System (HIS)

Neonatal Cardiomyocyte Isolation System (NCIS)

Papain Dissociiation System (PDS/PDS2)

艾美捷Worthington胶原蛋白酶原料认证：

粗胶原酶制剂含有两种不同胶原酶的几种同工型，种巯基蛋白酶、梭菌蛋白酶、种胰蛋白酶样酶和种氨肽酶。这种胶原蛋白水解和蛋白水解活性的组合可有效分解细胞间基质，这是组织解离的重要部分。该复合物的个成分是水解酶，它优先在序列 Pro-Y-Gly-Pro 中的 Y-Gly 键处降解天然胶原蛋白中的螺旋区域，其中 Y 常见的是中性氨基酸。这种切割产生对进步肽酶消化敏感的产物。粗胶原酶被半胱氨酸、EDTA 或邻菲咯啉等金属螯合剂抑制，但 DFP 不抑制。它也被种大的血浆糖蛋白α2-巨球蛋白抑制。钙2+是酶活性所必需的。每种胶原酶的特定酶谱已与从中获得研究细胞的组织（或与放置细胞的用途）相关，并且作为相关性的结果，Worthington 已经建立了几种类型的粗胶原酶：类型 1、2、3 和 4。

• 1 型粗胶原酶具有胶原酶、酪蛋白酶、梭菌蛋白酶和胰蛋白酶活性的原始平衡。

• 2 型含有较高相对水平的蛋白酶活性，尤其是梭菌蛋白酶。

• 3 型含有低水平的二蛋白酶。

• 4 型被设计成胰蛋白酶活性特别低，以限制对膜蛋白和受体的损害。

• 5 型 含有较高的胶原酶和酪蛋白酶值。

• 6 型 含有高胶原酶活性，酪蛋白酶与胶原酶的比例约为 2:1。设计为相对于 I 型 ( col  G)富含 II 型 ( col  H) 胶原酶。

• 7 型胶原酶和酪蛋白酶的活性比 1 型和 2 型胶原酶高四倍。

来源：https://www.amyjet.com/products/WBC-LS004188.shtml