EndoTrap is an affinity chromatography resin for the efficient removal of bacterial endotoxins

(lipopolysaccharides/LPS) from aqueous solutions containing low or high molecular weight substances such as proteins ａnd nucleic acids. EndoTrap can be employed both in column or batch mode, by gravity flow or on fully automated liquid chromatography systems.

The EndoTrap family contains two members:

EndoTrap HD

with a broad pH ａnd salt tolerance, works best with calcium or magnesium compatible buffers such as TRIS, HEPES, MOPS but also PBS, if enriched freshly with 1 mM Ca/Mg2+ ａnd 1 mM Citrate.

Furthermore, it was particularly developed for challenging samples ａnd large scale endotoxin

removal in e.g biopharmaceutical production processes. For EndoTrap HD, a Regulatory Support File (RSF) ａnd an EndoTrap leakage ELISA are available additionally.

EndoTrap red

especially for the use with buffers containing calcium chelators.

This multipurpose product portfolio covers a broad range of conditions with no special equipment or buffer requirements. EndoTrap HD 1/1 ａnd 5/1 as well as all EndoTrap red kits include all buffers needed. EndoTrap HD is supplied including instructions for buffer preparation ａnd can be supplied including buffers on request.

The following tables give a short overview on the differences of the EndoTrap systems.

艾美捷内毒素纯化树脂，是一种用于去除生物源蛋白类产品(包括多肽、抗体、多糖等)中内毒素的树脂。其原理是将修饰过的多粘菌素B连接至4%琼脂糖微球上，进行特异性去除内毒素，经纯化可将样品中内毒素降低至0.1EU/ml，且样品回收率高。

艾美捷内毒素纯化树脂特点：

1、该内毒素纯化树脂满足工业应用的所有要求，并且可以轻松集成到/在早期或后期纯化过程中。

2、High-DefinitionMatrix还可作为预填充柱用于小规模纯化。

3、在4-9的pH值范围和50-600mM的NaCl浓度范围内，使用EndoTrap®HD去除内毒素有效。

4、快速无孵育步骤–真正的流通系统，J佳蛋白质回收率将样品损失降至低，样品回收率高达99%，LPS变异性广泛菌株的内毒素去除，可重复使用/再生可重复使用至少三次，EndoTrap HD至少10次，内毒素去除效率损失；-包括再生缓冲液，再生物质无毒，无脱氧胆酸盐（DOC）!

*建议使用HEPES、TRIS、MOPS、MES和PIPES等缓冲液。必须避免使用柠檬酸盐缓冲液和二价阳离子螯合剂（如EDTA）

艾美捷内毒素纯化树脂EndoTrap HD和EndoTrap-red规格比较：

来源：https://www.amyjet.com/products/LET0023.shtml