Information

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion ａnd two antigen binding Fab portions joined together by disulfide bonds ａnd therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures ａnd is the most cost effective.

Usage

Physical State: Freeze-dried solid

Storage ａnd Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) ａnd centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.

Extended Storage after Rehydration: Aliquot ａnd freeze at -70°C or below. Avoid repeated freezing ａnd thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, ａnd store at -20°C as a liquid.

Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6

Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)

Preservative: None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)

Suggested Working Concentration or Dilution Range:

1:500 - 1:5,000 for immunohisto/cytochemistry

1:5,000 - 1:100,000 for ELISA ａnd Western blotting with chromogenic substrates

1:10,000 - 1:200,000 for Western blotting with ECL substrates

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

115-035-003 HRP标记亲和纯化山羊抗小鼠IgG（H+L）（艾美捷）中文说明：

基于免疫电泳和/或ELISA，抗体与全分子小鼠IgG反应。它还与其他小鼠免疫球蛋白的轻链反应。未检测到针对非免疫球蛋白血清蛋白的抗体。该抗体可能与其他物种的免疫球蛋白发生交叉反应。

通过免疫亲和层析从抗血清中分离出完整的IgG抗体。它们具有通过二硫键连接在一起的Fc部分和两个抗原结合Fab部分，因此它们是二价的。据报道，平均分子量约为160kDa。整个IgG形式的抗体适用于大多数免疫检测程序。

115-035-003辣根过氧化物酶HRP标记亲和纯化山羊抗小鼠IgG（H+L）基本参数：

目标：鼠

宿主：山羊

抗体形式：全IgG

特异性：IgG（H+L）

标记物：辣根过氧化物酶

类别：全IgG亲和纯化抗体

克隆性：多克隆

物理状态：冻干固体

储存和再水合：在2-8°C下储存冻干固体。用指定体积的dH2O重新水合，如果不清楚，则进行离心。使用当天准备工作稀释液。在2-8°C下作为未稀释液体稳定约6周。

脱水后的长期储存：等分并在-70°C或更低温度下冷冻。避免反复冷冻和解冻。或者，添加等量的甘油（ACS或更好），终浓度为50%，并以液体形式储存在-20°C。

纯度：抗体通过免疫亲和层析从抗血清中纯化，使用偶联到琼脂糖珠的抗原。

缓冲液：0.01MNa₃PO₄，0.25M NaCl，pH 7.6

稳定剂：15 mg/ml牛血清白蛋白（无IgG，无蛋白酶）

防腐剂：无

建议工作浓度或稀释范围：

1： 500-1:5000用于免疫组织/细胞化学

1： 5000-1:100000用于ELISA和显色底物的蛋白质印迹

1： 10000-1:200000用于ECL底物的蛋白质印迹

稀释因子以范围的形式表示，因为合适的稀释是许多因素的函数，如抗原密度、渗透性等。实际使用的稀释必须根据经验确定。

来源：https://www.amyjet.com/products/115-035-003.shtml