INTENDED USE：

The Human CP ELISA is a highly sensitive two-site enzyme-linked immunoassay (ELISA) for the quantitative

determination of CP in human biological samples. For research use only. Not for use in diagnostic procedures.

INTRODUCTION：

Ceruloplasmin is a multifunctional protein involved in copper transport ａnd is also an important serum antioxidant. During inflammation, traumatic injury, or myocardial infarction, the serum levels of ceruloplasmin, as an acute phase reactant,rise 2-3 fold.

PRINCIPLE：

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the CP present in samples reacts with the anti-CP antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-CP is added ａnd complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added ａnd complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’- tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CP in the test sample. The quantity of CP in the sample can be interpolated from the calibration curve constructed from the calibrators, ａnd corrected for sample dilution.   

近年来研究结果认为铜蓝蛋白起着抗氧化剂的作用。在血循环中铜蓝蛋白的抗氧化活力可以防止组织中脂质过氧化物和自由基的生成，特别在炎症时具有重要意义。铜蓝蛋白也属于一种急性时相反应蛋白。血浆铜蓝蛋白在感染、创伤和肿瘤时增加。其特殊的作用在于协助Wilson病的诊断，即患者血浆铜蓝蛋白含量明显下降，而伴有血浆可透析的铜含量增加。大部分患者可有肝功能损害并伴有神经系统的症状，如不及时治疗，此病是进行性和致命的。血浆铜蓝蛋白在营养不良、严重肝病及肾病综合征时亦往往下降。妇女妊娠期、口服避孕药时其含量有明显增加。

艾美捷KT-1876人铜蓝蛋白ELISA试剂盒是一种高度灵敏的双位点酶联免疫分析（ELISA）人类生物样品中CP的测定。仅供研究使用。不用于诊断程序。

人铜蓝蛋白ELISA试剂盒组成：

1.浓缩稀释剂

一瓶含有50mL 5X浓缩稀释液流动缓冲液。

2.浓缩洗涤液

一瓶含有50mL 20X浓缩洗涤液。

3.检测抗体100X

一个小瓶含有150µL亲和纯化的抗人CP抗体，该抗体在稳定缓冲液中与生物素缀合。

4.HRP链亲和素100X

一个小瓶，在稳定缓冲液中含有150µL HRP结合的链霉亲和素。

5.TMB基板溶液

一瓶含有12mL TMB和过氧化氢的柠檬酸缓冲液（pH 3.3）。

6.S Solution

一瓶含12 mL 0.3 M硫酸。

警告：避免接触皮肤。

7.微量滴定板

井支架框架中的十二个可拆卸的八（8）个井微孔条。每个孔用亲和纯化的抗人CP包被。

8.人体CP校准器

一个装有冻干人CP校准品的小瓶。

人铜蓝蛋白ELISA试剂盒标本采集和处理：

应通过静脉穿刺收集血液，并在凝块形成后通过离心将血清与细胞分离。对于血浆样本，应将血液收集到装有抗凝剂的容器中，然后离心。应注意为了尽量减少溶血，过量的溶血会影响您的结果。立即测定或等分并储存-20°C下的样品。避免重复冻融循环。

对于任何可能含有病原体的样本，必须小心防止与开放性伤口接触。无添加剂或防腐剂是保持标本完整性所必需的。避免叠-氮-化物污染。

来源：https://www.amyjet.com/products/KT-1876.shtml