DH2 to be used for 14,15-EET/DHET measurement is the same kit as DH1 for measurement of 14,15-DHET. The only difference with DH2 compared with DH1 is the sample preparation step (NOT ELISA kit) in which EET is chemically changed to DHET.

The 14,15-DHET is a representative metabolite of soluble epoxide hydrolase (sEH)-mediated metabolism of EETs, generated by arachidonic acid epoxygenase activity of cytochromes P450 (CYPs) 2C ａnd 2J. This competitive ELISA kit with an HRP system has been used to determine 14,15-DHET levels in biological samples (tissue,plasma ａnd urine) ａnd cell culture media. Using the 14,15-DHET ELISA, strong positive correlation has been discovered with 14,15-DHET levels on hypertension ａnd brain injury ａnd stroke, ａnd EET levels on a metastatic phenotype of carcinoma cells ａnd insulin resistance. Next generation hypertension drug discoveries have been carried out at several laboratories. Each kit for a 96-well plate is good for triplicate analyses of up to 24 samples.

14，15-DHET是可溶性环氧化物水解酶（sEH）介导的EET代谢的代表性代谢产物，由细胞色素P450（CYPs）2C和2J的花生四烯酸环氧合酶活性产生。这种竞争具有HRP系统的ELISA试剂盒已用于测定生物样品（组织，血浆和尿液）和细胞培养基。使用14,15-DHET-ELISA，已发现强正相关发现高血压、脑损伤和中风患者的14,15-DHET水平，转移性脑脊髓炎患者的EET水平癌细胞表型和胰岛素抵抗。下一代高血压药物的发现在几个实验室进行。96孔板的每个试剂盒适用于多24个样品的一式三份分析。

（左）自发性高血压大鼠尿14,15-DHET水平升高。

（右）妊娠高血压（先兆子痫）妇女的尿14,15-DHET水平显著与健康孕妇在98%置信度下的差异（p＜0.02）

艾美捷14,15-DHET Hypertension ELISA#DRD-DH2部分参考文献：

1. Kim J, Yoon SP, Toews ML, Imig JD, Hwang SH, Hammock BD, Padanilam BJ. Pharmacological inhibition of soluble epoxide hydrolase e prevents renal interstitial fibrogenesis in obstructive nephropathy. Am J Physiol Renal Physiol. 2015 Jan 15;308(2):F131-9. doi: 10.1152/ajprenal.00531.2014. PMID: 25377915.

2. Mattace Raso G, Pirozzi C, d＇Emmanuele di Villa Bianca R, Simeoli R, Santoro, A, Lama A, Di Guida F, Russo R, De Caro C, Sorrentino R, Calignano A, Meli R. Palmitoylethanolamide treatment reduces blood pressure in spontaneously hypertensive rats: involvement of cytochrome p450-derived eicosanoids ａnd Renin Angiotensin system. PLoS One. 2015 May 7;10(5):e0123602. doi: 3.1371/journal.pone.0123602. eCollection 2015. PMID: 25951330.

10. Dai M, Wu L, He Z, Zhang S, Chen C, Xu X, Wang P, Gruzdev A, Zeldin DC, Wang DW. Epoxyeicosatrienoic Acids Regulate Macrophage Polarization ａnd Prevent LPS-Induced Cardiac Dysfunction. J Cell Physiol. 230(9):2108-19. 2015 doi: 10.1002/jcp.24939. PMID: 25626689.

14,15-DHET Hypertension ELISA相关研究：

14,15-DHET Immunoassay Kit     DH1

BioTarget 14,15-DHET Human Urine ELISA Kit DH3

14,15-DHET Immunoaffinity Column for One Step Purification     DH31

14,15-DHET sEH activity ELISA Kit    SH1

来源：https://www.amyjet.com/products/BXC-BE0045-1mg.shtml