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lucigen 40300-4 40300-2 CloneSmart® LCAmp Blunt Clon
点击次数：0发布时间：2019/7/16 15:39:26
更新日期：2019/7/16 15:39:26
所 在 地：中国大陆
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lucigen 40300-4 40300-2 CloneSmart® LCAmp Blunt Cloning Kit
产品名称：CloneSmart® LCAmp Blunt Cloning Kit</p>
货号：lucigen 40300-4</p>
规格：40 rxns</p>
存储温度：-20º C</p>
产品名称：CloneSmart® LCAmp Blunt Cloning Kit</p>
货号：lucigen 40300-2</p>
规格：20 rxns</p>
存储温度：-20º C</p>
品牌：lucigen</p>
供应商：中北林格</p>
产地：us</p>
发货地：北京</p>
lucigen 40300-4 40300-2 CloneSmart® LCAmp Blunt Cloning Kit 产品说明书
CloneSmart® Cloning Kits (pSMART® Vectors)
- Convenient, pre-processed vectors eliminate the need for digestion, gel purification, ａnd dephosphorylation.
- Transcription terminators flanking cloning site stabilize otherwise toxic inserts.
- Choice of copy number ａnd antibiotic resistance.
- Optimized for use with E. cloni® 10G electrocompetent and chemically competent cells (sold separately)
Clone quickly ａnd facilitate downstream applications
Lucigen’s unique cloning kits with pre-processed vectors ａnd highly competent cells eliminate hours of tedious preparation ａnd enable efficient cloning. Ligation can be completed in as little as 30 minutes with no clean-up needed; only a brief heat denaturation is necessary before transformation. Downstream manipulation (e.g, mutagenesis, subsequent ｉnsert addition) is facilitated by the minimal vector size (1.7-2.0 kb).
Stabilized Inserts ａnd Gap-Free Cloning
All cloning kits include the transcription- ａnd translation-free pSMART® vectors (Figure 1), which eliminate many of the problems associated with cloning recalcitrant DNA in conventional plasmid vectors. Conventional cloning vectors contain promoters (e.g., lacZ promoter) that constitutively transcribe the ｉnsert sequence. This transcription ａnd coupled translation can destabilize inserts that contain coding regions, strong promoters, short repeats, or incompatible secondary structures. The cloning site of pSMART vectors does not include a lacz sequence. In addition, strong transcription terminators flank the cloning site to block spurious transcription from the vector ａnd insert-driven transcription into the vector. Low copy number versions of pSMART further stabilize sequences that are difficult to maintain in typical vectors.
No Background Problems
The pSMART vectors are supplied pre-digested, with blunt, dephosphorylated ends, ａnd are qualified to produce 99.5% recombinant clones in typical experiments. The ultra-low background of empty vector (less than 0.5%) eliminates the need to screen for recombinants, removes the uncertainty of false negatives (light blue pUC colonies) ａnd false positives (white colonies that lack inserts), ａnd enables library construction from nanogram amounts of DNA.
Figure 1. High copy (HC) ａnd low copy (LC) versions of the pSMART transcription-free cloning vectors.
Convenient Success
Efficiently obtain your clone or create your genomic or cDNA library—the CloneSmart Cloning Kits eliminate tedious preparation of vectors ａnd competent cells, as well as time-consuming QC testing experiments. Kits include optimized reagents, ligation-ready vector (no post-ligation cleanup step required), detailed instructions, ａnd trouble-shooting guides to simplify cloning ａnd sequencing. Highly efficient E. cloni® 10G electrocompetent cells (up to >4 × 1010 cfu/µg) or chemically competent cells ( >1 × 109 cfu/µg) are available for sale separately.
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Resources
| Product Description | Size | Cat. No. | |
| CloneSmart HCAmp Blunt Cloning Kit | 20 rxns | 40041-2 | |
| 40 rxns | 40041-4 | ||
| CloneSmart HCKan Blunt Cloning Kit | 20 rxns | 40704-2 | |
| 40 rxns | 40704-4 | ||
| CloneSmart LCAmp Blunt Cloning Kit | 20 rxns | 40300-2 | |
| 40 rxns | 40300-4 | ||
| CloneSmart LCKan Blunt Cloning Kit | 20 rxns | 40821-2 | |
| 40 rxns | 40821-4 | ||
CloneSmart Blunt Cloning Kits contain: Vector Premix, CloneSmart DNA Ligase, Positive Control Insert DNA, Sequencing Primers, ａnd a complete protocol. Kits can be used with any E. coli chemically competent or electrocompetent cells but have been optimized for use with of E. cloni® Electrocompetent Cells and Chemically Competent Cells.
CloneSmart®克隆试剂盒（pSMART®载体）
- 方便的预处理载体消除了消化，凝胶纯化和去磷酸化的需要。
- 侧翼克隆位点的转录终止子稳定了有毒的插入物。
- 选择拷贝数和抗生素抗性。
- 优化用于 E.cloni®10G 电感受态和化学感受态细胞（另售）
快速克隆并促进下游应用
Lucigen独特的克隆试剂盒具有预处理载体和高效细胞，可消除繁琐的繁琐制备并实现高效克隆。结扎可在短短30分钟内完成，无需清理; 转化前只需要短暂的热变性。通过*小载体大小（1.7-2.0kb）促进下游操作（例如，诱变，随后的插入添加）。
稳定插入和无间隙克隆
所有克隆试剂盒都包括无转录和翻译的pSMART®载体（图1），它消除了与常规质粒载体中克隆顽固DNA相关的许多问题。常规克隆载体含有组成型转录插入序列的启动子（例如，lacZ启动子）。这种转录和偶联翻译可以使含有编码区，强启动子，短重复或不相容的二级结构的插入物不稳定。pSMART载体的克隆位点不包括 lacz序列。此外，强大的转录终止子位于克隆位点的侧翼，以阻止来自载体的杂散转录和插入驱动的转录到载体中。pSMART的低拷贝数版本进一步稳定了在典型载体中难以维持的序列。
没有背景问题
pSMART载体预先消化，具有钝的去磷酸化末端，并且在典型实验中有资格产生99.5％重组克隆。空载体的超低背景（小于0.5％）消除了筛选重组体的需要，消除了假阴性（浅蓝色pUC菌落）和假阳性（缺少插入物的白色菌落）的不确定性，并使图书馆构建成为可能。纳克量的DNA。
图 1.pSMART无转录克隆载体的高拷贝（HC）和低拷贝（LC）版本。
方便成功
有效地获得克隆或创建基因组或cDNA文库 - CloneSmart克隆试剂盒消除了繁琐的载体和感受态细胞的制备，以及耗时的QC测试实验。试剂盒包括优化的试剂，准备连接的载体（无需连接后的清理步骤），详细说明和故障排除指南，以简化克隆和测序。高效 E.cloni®10G电感受态细胞（> 4×1010 cfu /μg）或化学感受态细胞（> 1×109 cfu /μg）可单独出售。
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资源
| 产品描述 | 规格 | 货号 | |
| CloneSmart HCAmp Blunt Cloning Kit | 20 rxns | 40041-2 | |
| 40 rxns | 40041-4 | ||
| CloneSmart HCKan Blunt Cloning Kit | 20 rxns | 40704-2 | |
| 40 rxns | 40704-4 | ||
| CloneSmart LCAmp钝克隆试剂盒 | 20 rxns | 40300-2 | |
| 40 rxns | 40300-4 | ||
| CloneSmart LCKan Blunt Cloning Kit | 20 rxns | 40821-2 | |
| 40 rxns | 40821-4 | ||
CloneSmart Blunt Cloning Kits包含：Vector Premix，CloneSmart DNA连接酶，阳性对照插入DNA，测序引物和完整的方案。 试剂盒可与任何 大肠杆菌化学感受态或电感受态细胞一起使用，但已经过优化，可与 E.cloni®ElectrocompetentCells 和Chemically Competent Cells一起使用。
