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应用领域说明
- RNA聚合酶抑制研究。
- RNA polymerase inhibition studies.
- Transcription studies.
- 转录研究。
Tagetin™RNA聚合酶抑制剂是*早发现的一种引起绿化的细菌性植物毒素,它是已知能有效和选择性抑制多种真核生物体(包括哺乳动物细胞,酿酒酵母, 果蝇果蝇, 家蚕和 非洲爪蟾)中的 RNA聚合酶III的化合物。 拉维斯卵母细胞。1 它强烈抑制 大肠杆菌 RNA聚合酶和植物叶绿体RNA聚合酶。2 植物核RNA聚合酶I,II和III对Tagetin抑制剂的敏感性低得多。噬菌体编码的RNA聚合酶(例如SP6和T7)也相对不敏感。3 对于真核和原核RNA聚合酶,抑制程度均取决于模板。4 Tagetin抑制剂可补充α-amanitin的活性,α-amanitin是一种有效的选择性真核RNA聚合酶II抑制剂。尽管尚不清楚确切的抑制机理,但对酵母核提取物的研究表明,其作用是由于模板上离散点上的延伸复合物暂停增加所致。4
First discovered as a bacterial phytotoxin responsible for inducing chlorosis, Tagetin™ RNA Polymerase Inhibitor is the only compound known to potently and selectively inhibit RNA polymerase III from a variety of eukaryotic organisms including mammalian cells, Saccharomyces cerevisiae, Drosophila melanogaster, Bombyx mori, and Xenopus laevis oocytes.1 It strongly inhibits Escherichia coli RNA polymerase and plant chloroplast RNA polymerase.2 Plant nuclear RNA polymerases I, II, and III are much less sensitive to Tagetin Inhibitor. Phage-encoded RNA polymerases such as SP6 and T7 are also relatively insensitive.3 With both eukaryotic and prokaryotic RNA polymerases, the degree of inhibition is template-dependent.4 Tagetin Inhibitor complements the activity of α-amanitin, a potent and selective inhibitor of eukaryotic RNA polymerase II. Although the precise mechanism of inhibition is not understood, studies with yeast nuclear extracts indicate that the effect is due to increased pausing of the elongation complex at discrete points on the template.4好处说明
- 来自许多非植物真核生物的已知的RNA聚合酶III特异性抑制剂。
- 特异性抑制许多植物的叶绿体RNA聚合酶。
- Only known specific inhibitor of RNA polymerase III from many nonplant eukaryotes.
- Specific inhibition of chloroplast RNA polymerase of many plants.
单位定义: 在噬菌体T7 DNA为模板的标准测定条件下,一单位(30 pmol)的Tagetin抑制剂可抑制50%抑制1单位的 大肠杆菌 RNA聚合酶全酶。
Unit Definition: One unit (30 pmol) of Tagetin Inhibitor results in 50% inhibition of 1 unit of E. coli RNA Polymerase Holoenzyme under standard assay conditions using phage T7 DNA as a template.
储存缓冲液: 水中提供的Tagetin RNA聚合酶抑制剂浓度为20 U / µl(600 µM)。
Storage Buffer: Tagetin RNA Polymerase Inhibitor is provided in water at a concentration of 20 U/µl (600 µM).
质量控制: Tagetin抑制剂已通过EPICENTRE的大肠杆菌 RNA聚合酶全酶进行了功能测试, 并且没有可检测的RNase和DNase活性。
Quality Control: Tagetin Inhibitor is functionally tested with EPICENTRE's E. coli RNA Polymerase Holoenzyme and is free of detectable RNase and DNase activities.
图1. Tagetin抑制剂对大肠杆菌 RNA聚合酶的活性 。 每个凝胶泳道显示了使用噬菌体模板,1 U的大肠杆菌 RNA聚合酶全酶和不同量的Tagetin抑制剂的标准转录反应 的产物。1巷,100 U; 2巷10 U; 3巷,1 U; 泳道4,0.1 U; 第5泳道,无他汀汀抑制剂的对照。在每单位大肠杆菌 RNA聚合酶1 U Tagetin抑制剂处观察到50%的转录抑制 。