产品简介：

          荧光标记细胞已被广泛证实可有效确定总细胞的数量和一个样本中存在多少活细胞。流式细胞术结合荧光染色法是分析非均质细胞种群的有力工具。非荧光性的FDA和它的衍生物分子进入细胞后被胞内无特异性的酯酶所水解产生荧光。这些荧光产物只能积聚在具有完整细胞膜的细胞中。因此，死细胞无完整细胞膜不能被染色。FDA及其类似物（如CDCFDA）精细的跨膜动力学以及胞内水解特点与细胞功能相关，因此FDA标记可以通过荧光显微镜或者流式细胞仪来检测细胞。不过FDA荧光标记的细胞在细胞系或者菌株中存在变化，可能是由于不同胞内酯酶的活性所造成的。CDCFDA是一个胺化FDA衍生物，可以用来制备各种FDA偶联物。

英文注释：

It is widely recognized that fluorescent labeling of cells is an effective means to determine total cell numbers or how many viable cells exist in a sample. Flow cytometry combined with fluorescent staining is a powerful tool to analyze heterogeneous cell populations. Fluorescein diacetate （FDA） ａnd its derivatives are non-fluorescent molecules that diffuse into cells ａnd are hydrolyzed by intracellular non-specific esterases to give fluorescent products. The fluorescent products can be accumulated only in those cells that have intact cell membranes; therefore, dead cells with leaky membranes are not stained. The precise kinetics of membrane transport ａnd intracellular hydrolysis of FDA ａnd its analogs （such as CDCFDA） are related to cellular functions, thus FDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The fluorescence intensity of labeled cells by FDA dyes varies considerably among cell lines ａnd strains, probably because of differences in intracellular esterase activity. CDCFDA is an amine杛eactive FDA derivative that can be used to prepare a variety of FDA conjugates.