产品展示
Amplite 红色荧光底物
点击次数:34发布时间:2014/10/14 10:17:22
更新日期:2014/11/24 10:18:01
所 在 地:中国大陆
产品型号:11011
优质供应
详细内容
产品简介:
我们Amplite?红色是具有571 nm和585 nm的发射吸收高度红色荧光产品的敏感荧光过氧化物酶底物。不像其他的HRP底物如dihydrofluoresceins和dihydrorhodamines,Amplite?红色是空气中氧化*小的。Amplite?红色是*敏感的,稳定的荧光探针,用于检测HRP和H2O2。 Amplite?红在许多免疫测定中已被广泛用于检测HRP。另一方面,Amplite?红也可用于检测H2O2的微量。该Amplite?红色H2O2检测幅值中scopoletin测定法*为敏感。因为H2O2是产生于许多酶的氧化还原反应,Amplite?红色可以在耦合酶促反应中用于检测多种氧化酶和/或相关的酶/底物或辅因子,如葡萄糖,乙酰胆碱和胆固醇,L-谷氨酸,氨基酸的活性等。
英文注释:
Our Amplite Red is a sensitive fluorogenic peroxidase substrate that generates a highly red fluorescent product that has maximum absorption of 571 nm and maximum emission of 585 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of Amplite Red is minimal. Amplite Red is one of the most sensitive and stable fluorogenic probes for detecting HRP and H2O2. Amplite Red has been widely used to detect HRP in many immunoassays. On the other hand, Amplite Red can also be used to detect trace amount of H2O2. The Amplite Red-based H2O2 detection is at least one order of magnitude more sensitive than the commonly used scopoletin assay for H2O2. Because H2O2 is produced in many enzymatic redox reactions, Amplite Red can be used in coupled enzymatic reactions to detect the activity of many oxidases and/or related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol, L-glutamate, amino acids, etc.