企业档案
- 会员类型:免费会员
- 工商认证: 【已认证】
- 最后认证时间:
- 法人:
- 注册号:
- 企业类型:生产商
- 注册资金:人民币万
联系我们
联系人:杨燕燕
热门标签
技术文章
推荐:Type I Collagen Solution, 3 mg/ml (Human)
Catalog #5007
VitroCol® is a 3 mg/ml, type I human atelocollagen solution for 2D coatings, 3D hydrogels, or as a collagen standard. VitroCol® collagen is naturally secreted from human neo-natal fibroblast cells, then processed and purified to yield the naturally produced human collagen.Product DescriptionVitroCol® collagen is the first widely available, naturally produced purified human collagen for research purposes. VitroCol® sets the standard for purity ( 99% collagen content), functionality and represents the only native-like human collagen offered.
VitroCol® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblast to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen.
VitroCol® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. VitroCol® is supplied at approximately 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. VitroCol® is soluble atelocollagen in 0.01 N HCI, therefore, the pH is 2.
VitroCol® is especially ideal for human cell culture systems when coating of surfaces, providing preparations of thin layers of culturing cells, or use as a solid gel. VitroCol® human collagen is provided in user-friendly packaging for use and storage. VitroCol® is sterile filtered and is supplied as a ready to use solution.
Parameter, Testing, and Method
VitroCol® Type I Collagen #5007

推荐:Type I Collagen Solution, 3 mg/ml (Human)
Sterilization Method
Filtration
Extraction Method
Enzyme - atelocollagen
Form
Solution
Package Size
20 mL, 100 mL
Storage Temperature
2-10°C
Shelf Life
Minimum of 6 months from date of receipt
Collagen Concentration - Biuret
2.9-3.2 mg/mL
Collagen Purity - Silver Staining
99%
pH
1.9-2.1
Kinetic Gel Test (Minutes)
40
Gel Formation Tube Test (Minutes)
40
Fibrillogenesis (Absorbance Units)
0.5
Electrophoretic Pattern - Coomassie Blue
Characteristic
Sterility - USP modified
No growth
Endotoxin - LAL
5.0 EU/mL
Osmolality (mOsmo H2O/kg)
35
Cell Attachment Assay
Pass
Source
Human Neo-Natal Fibroblasts
Hydrogel Young s Modulus E (Pa)
Characteristic
Product ReferencesReferences for VitroCol®:
Shieh, Hester F., et al. Comparisons of human amniotic mesenchymal stem cell viability in FDA-approved collagen-based scaffolds: Implications for engineered diaphragmatic replacement. Journal of pediatric surgery 52.6 (2017): 1010-1013.
van der Velden, Jos LJ, et al. Transforming Growth Factor-? Induces A Mesenchymal Profile In Human Nasal Epithelial Cells. D76. ALVEOLAR EPITHELIUM: NOVEL TOOLS AND PHENOTYPES. American Thoracic Society, 2012. A6322-A6322.
Tashima, Takumi, et al. Osteomodulin regulates diameter and alters shape of collagen fibrils. Biochemical and biophysical research communications 463.3 (2015): 292-296.
Spiller, Kara L., Suzanne A. Maher, and Anthony M. Lowman. Hydrogels for the repair of articular cartilage defects. Tissue engineering part B: reviews 17.4 (2011): 281-299.
Brilha, Sara, et al. Monocyte adhesion, migration, and extracellular matrix breakdown are regulated by integrin αVβ3 in Mycobacterium tuberculosis infection. The Journal of Immunology 199.3 (2017): 982-991.
Jonsdottir, Hulda R., and Ronald Dijkman. Characterization of human coronaviruses on well-differentiated human airway epithelial cell cultures. Coronaviruses. Humana Press, New York, NY, 2015. 73-87.
Sabbione, Florencia, et al. Neutrophil extracellular traps stimulate proinflammatory responses in human airway epithelial cells. Journal of innate immunity 9.4 (2017): 387-402.
Dos Santos Brilha, S., et al. Monocyte adhesion, migration and extracellular matrix breakdown is regulated by integrin αVβ3 in Mycobacterium tuberculosis infection.
Brilha, Sara, et al. Monocyte Adhesion, Migration, and Extracellular Matrix Breakdown Is Regulated by Integrin aVb3 in Mycobacterium tuberculosis Infection. (2017).
Colace, T., et al. Analysis of morphology of platelet aggregates formed on collagen under laminar blood flow. Annals of biomedical engineering 39.2 (2011): 922-929.
Muthard, Ryan W., and Scott L. Diamond. Blood clots are rapidly assembled hemodynamic sensors: flow arrest triggers intraluminal thrombus contraction. Arteriosclerosis, thrombosis, and vascular biology 32.12 (2012): 2938-2945.
Maloney, S. F., Lawrence F. Brass, and S. L. Diamond. P2Y12 or P2Y1inhibitors reduce platelet deposition in a microfluidic model of thrombosis while apyrase lacks efficacy under flow conditions. Integrative Biology 2.4 (2010): 183-192.
Bauer, Rebecca N., et al. Interaction with epithelial cells modifies airway macrophage response to ozone. American journal of respiratory cell and molecular biology 52.3 (2015): 285-294.
Kawamura, Shunsuke, et al. Identification of common monocyte progenitors, pre-monocytes, and granulocyte monocyte progenitors in human umbilical cord blood. Experimental Hematology 43.9 (2015): S72.

IF<5,赠送价值 500元礼品卡
5 ≤IF≤10,赠送价值 1000元礼品卡
IF≥10,赠送价值 1000元礼品卡+任意 1 款 ValukineTM 试剂盒
DUMABIO ELISA试剂盒文献征集奖励活动
为感谢各位老师对笃玛品牌的支持与厚爱,凡购买DUMABIO ELISA试剂盒,在SCI期刊杂志发表文中注明ELISA试剂盒采购上海笃玛生物科技有限公司(Shanghai DuMa Biotechnology Co., Ltd.)
Shanghai DuMa Biotechnology Co., Ltd.
发表刊物 影响因子 奖励金额
SCI期刊杂志 1.0≤1F<3.0 200元
SCI期刊杂志 3.0≤1F<6.0 400元
SCI期刊杂志 6.0≤1F<10.0 600元
SCI期刊杂志 1F≥10 1000元
原创作者:上海笃玛生物科技有限公司