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技术文章
推荐：Beta-Amyloid (1-42)
中文名： β-淀粉样多肽 1-42 | Beta-淀粉样多肽 1-42 | Beta-淀粉样多肽 -42 | Β-淀粉样多肽(1-42) | Beta-Amyloid多肽(1-42)
纯度： >95% by HPLC
序列(单字母)： DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
(三字母序列)： Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile- Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile-Ala
CAS No.： 107761-42-2
分子式： C203H311N55O60S
分子量： 4514.14
外观： 白色粉末
等电点： 5.2
净电荷(PH=7)： -2.7
平均亲水性： -0.1
亲水残基比例： 31%
盐： TFA
来源： 化学合成
储存条件(冻干粉)：
建议冻干粉在-20℃或-80℃冷冻干燥保存。
由于多肽的每次冻融均会引起部分失活，如果需要液体保存，须分装后-20℃或-80℃冻存，以避免反复冻融，建议2-4周内用完。
溶解条件： Acetic acid/water (3:2) or DMSO
运输条件： 常温（加冰袋）
描述： Alzheimer＇s disease is a common neurodegenerative, progressive, ａnd fatal disorder. Generation ａnd deposition of amyloid beta (Aβ) peptides associate with its pathogenesis ａnd small soluble Aβ oligomers show the most pronounced neurotoxic effects ａnd correlate with disease initiation ａnd progression.
Beta amyloid 1-42 is a 42-amino acid peptide which plays a key role in the pathogenesis of Alzheimer, even slightly increased amounts of this Aβ(1–42) might be sufficient to cause Alzheimer＇s disease (AD).
推荐：Beta-Amyloid (1-42)
Beta amyloid proteins 聚集指南（以下方案仅供参考，请根据您的具体实验需求进行调整）:
1. 将Aβ肽(1-42)粉末溶于冷的六氟-2-丙醇（HFIP）。肽溶液在室温下孵化至少1小时，以建立单体化和随机化的结构。(Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization ａnd randomization of structure.)
2. 蒸发掉HFIP，将得到的多肽以薄膜形式保存在-20或-80℃。(The HFIP was removed by evaporation, ａnd the resulting peptide was stored as a film at -20 or -80 ℃.)
3. 将得到的薄膜以5 mM的浓度溶解于无水DMSO中，然后用涡旋方式稀释到适当的浓度和缓冲液（不含血清和酚红的培养基）中。(The resulting film was dissolved in anhydrous DMSO at 5 mM ａnd then diluted into the appropriate concentration ａnd buffer (serum- ａnd phenol red-free culture medium) with vortexing.)
4. 接下来，该溶液在4-8℃下老化48h。然后在4-8 ℃下以14000g离心10分钟；可溶性低聚物在上清液中。将上清液稀释10-200倍用于后续实验。(Next, the solution was age 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments.)
以上方法因后续不同应用而异(Methods vary depends on the downstream applications.)。
注意：老化后由于在溶液中聚集形态不稳定，建议现配现用。
原创作者：上海笃玛生物科技有限公司
