企业档案

  • 会员类型:免费会员
  • 工商认证: 【已认证】
  • 最后认证时间:
  • 法人:
  • 注册号:
  • 企业类型:生产商
  • 注册资金:人民币万

联系我们

联系人:杨燕燕

点击查看联系方式

技术文章

查找:rh-TNF-Alpha(rh-Tumor Necrosis Factor alpha)

点击次数:23 发布时间:2024/1/22 11:56:22
 查找:rh-TNF-Alpha(rh-Tumor Necrosis Factor alpha)

 

 

 

 

产品别名: AGS 1; AGS1; AGS-1; Aicardi Goutieres syndrome 1; ATIP; ATM and Rad3 related interacting protein; ATM and Rad3-related-interacting protein; ATR interacting protein; ATR-interacting protein; Atrip; ATRIP_HUMAN; Deoxyribonuclease III dnaQ/mutD (E. coli) like; DKFZp434J0310; DKFZp762J2115; DNase III; DRN 3; DRN3; FLJ12343; MGC20625; MGC26740; Three prime repair exonuclease 1; TREX 1; TREX1; TREX1 protein; MGC21482; 3' repair exonuclease 1; 3'-5' exonuclease TREX1.
英文名称:Anti-ATRIP/TREX1
抗体来源:Rabbit Or Mouse
性 状:Lyophilized or Liquid(冻干粉或液体)
浓 度:1mg/1ml
亚 型:IgG
产品规格:0.1ml/0.2ml
研究域:心,免疫学,等
交叉反应:人,大鼠,小鼠,犬,猪,羊等(Human, Mouse, Rat, Dog, Pig, Cow)
产品应用:WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

 

 

 

查找:rh-TNF-Alpha(rh-Tumor Necrosis Factor alpha)


 

 

 

 

 

注意事项:
1.重复冷冻/融化循环可使抗体变性,导致其形成使抗体结合能力降低的聚集物。保存于-20℃对于大多数抗体是适当的;保存于-80℃无明显优势。冰箱不能为无霜型。这些冷冻和融化之间的循环(以减少结霜)恰恰是应该避免的。
2.抗体试剂瓶应置于具有*小温度波动的冰箱区域,例如朝冰箱后部放置而不是置于门架上。有些研究人员加入冷冻保护剂甘油达到50%的终浓度以防止冷冻/融化损伤;甘油可将凝固点降低至低于-20℃。虽然这对于很多抗体是可接受的,但是只有一小部分我们提供的抗体测试了在该保存条件下的稳定性,并且我们的保证仅使用于按数据表上的方式保存的抗体。不建议将包含甘油的溶液保存于-80℃,因为该温度低于甘油的凝固点。请注意甘油可被细菌污染,如果加入甘油或任何冷冻保护剂,则应当小心操作,以得到无菌制备物。
3.应避免抗体在稀释至工作浓度后在4℃下保存超过一天。蛋白质在高浓度下保存时一般不易降解,理想的浓度为1mg/ml或更高。这是在抗体溶液中加入蛋白质例如BSA作为稳定剂的理论基础。加入的蛋白质也有助于减少抗体因结合于容器壁上而造成的损失。对于打算进行偶联的抗体而言,由于稳定蛋白质会与抗体竞争,降低了偶联的效率,所以不应加入稳定蛋白质。
温馨提示:
在实验后,请注意回收稀释的抗体。回收的抗体在进行Western实验时至少可以重复使用10次。稀释后的抗体,包括已经使用过的稀释抗体,4℃保存。回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。

 

 

 

选购指南:rh-TNF-Alpha(rh-Tumor Necrosis Factor alpha) 重组人Control system in WB experiment of antibody products:
Blotting Western (immunoblotting), (Western) is also known as protein imprinting (blotting), which is based on the specificity of antigen antibody binding to detect the complex sample of a certain protein. This method is a new kind of immune technology based on gel electrophoresis and solid phase immunoassay. Because of the high specificity and sensitivity of SDS-PAGE, the high specificity and sensitivity of the determination of the high specificity and sensitivity of the determination of the high specificity and sensitivity, has now become a common technique for protein analysis. Western blot is used to identify a protein, and it can be used for qualitative and semi quantitative analysis.
WB control system: the positive control: the best standard, or positive serum, positive supernatant.
In the negative control, the serum was used to measure the serum of the mice, and the culture supernatant was measured with a non clonal culture supernatant. Blank control: no one anti 1%BSA instead. Unrelated control (alternative control): antibodies to an unrelated item, or unrelated to the project.

 

 


原创作者:上海笃玛生物科技有限公司

相关产品

script>