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技术文章

ALPL Antibody (Center) Catalog Number:OM226338

点击次数:36 发布时间:2024/9/18 14:34:16
 

Product Profile

Product Name ALPL Antibody (Center)
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WBIFFC

 
WB~~1:100~500 IF~~1:10~50 FC~~1:10~50:
Species Reactivity

Human

Concentration 1 mg/ml
Purification  

Target Information

Alternative Names
ALPL
Alkaline phosphatase
tissue-nonspecific isozyme
Alkaline phosphatase liver/bone/kidney isozyme
More 
Molecular Weight(MW) 57305 Da
Function
This isozyme may play a role in skeletal mineralization.
Tissue Specificity This ALPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224~254 amino acids from the Center region of human ALPL.
Cellular Localization Cell membrane; Lipid-anchor, GPI-anchor.

Database Links

Entrez Gene 249

Application

  • Application

    Western blot analysis of ALPL (arrow) using rabbit polyclonal ALPL Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALPL gene.

  • Application

    ALPL Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the ALPL antibody detected the ALPL protein (arrow).

  • Application

    Confocal immunofluorescent analysis of ALPL Antibody (Center)(Cat#AP1474c) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

  • Application

    ALPL Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application Notes
WB~~1:100~500 IF~~1:10~50 FC~~1:10~50:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

原创作者:上海笃玛生物科技有限公司

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