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技术文章

Fibrinogen gamma antibody Catalog Number:OM253134

点击次数:55 发布时间:2024/9/19 14:18:16
 

Product Profile

Product Name Fibrinogen gamma antibody
Antibody Type Primary Antibodies
Product description
Mouse monoclonal antibody [4H9] to Fibrinogen gammaThe protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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Immunogen
The immunogen corresponding to a region within amino acids 210 and 437 of human FGG.

Key Feature

Clonality Monoclonal
Isotype IgG2a
Clone Number 4H9
Host Species Mouse
Tested Applications

ELISAFACSICC/IFIHC-PWB

 
Recommended Starting Dilutions: ELISA: 1/10000. WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. ICC/IF: 1/200 - 1/1000. FACS: 1/200 - 1/400. Not yet tested in other applications. Determining optimal working dilutions by titration test:
Species Reactivity

Human

Concentration 1 mg/ml
Purification  

Target Information

Gene Synonyms
FGG
Target Fibrinogen gamma
Molecular Weight(MW) 52 kDa (note)

Application

  • Application

    WB analysis of human Fibrinogen gamma recombinant protein (aa. 210-437) using Fibrinogen gamma antibody [4H9].

  • Application

    ELISA analysis of control antigen (100ng) and antigen using Fibrinogen gamma antibody [4H9].

  • Application

    FACS analysis of HepG2 cells using Fibrinogen gamma antibody [4H9] (blue) and negative control (red).

  • Application

    ICC/IF analysis of 3T3-L1 cells using Fibrinogen gamma antibody [4H9] (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Application

    IHC-P analysis of cerebellum tissues using Fibrinogen gamma antibody [4H9].

  • Application

    IHC-P analysis of liver cancer tissues using Fibrinogen gamma antibody [4H9].

Application Notes
Recommended Starting Dilutions: ELISA: 1/10000. WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. ICC/IF: 1/200 - 1/1000. FACS: 1/200 - 1/400. Not yet tested in other applications. Determining optimal working dilutions by titration test:

Additional Information

Form Liquid
Storage Instructions 4°C; -20°C for long term storage
Storage Buffer Ascitic fluid containing 0.03% sodium azide.

原创作者:上海笃玛生物科技有限公司

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