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技术文章
alpha Tubulin antibody (ascites) Catalog Number：OM239218
Product Profile
| Product Name | alpha Tubulin antibody (ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Purified chick brain tubulin. |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | DM1A |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PIPWBIB |
| IF: Use at a dilution of 1/500, this is determined by indirect immunofluorescent labeling of cultured chicken fibroblasts. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 50 kDa. Optimal dilutions/concentrations should be determined by the end user.: More | |
| Species Reactivity | HumanMouseAmphibiansFishFungiHamsterBovine |
| Concentration | 1 mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Synonyms | More |
|---|---|
| Target | alpha Tubulin |
| Cellular Localization | Major constituent of microtubules. |
Application
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Application
Chinese hamster embryonic fibroblasts in anaphase of mitosis were stained using Monoclonal Anti-α-Tubulin, clone no. DM1A with Anti-Mouse-Alexa 488 (green) ａnd DAPI (blue).
-
Application
Confocal image of the mouth of the Cryptocaryon irritans, a parasite of marine fishes, (theront stage) stained with Monoclonal Anti-α-Tubulin, clone no. DM 1A ａnd Anti-Mouse IgG-lissamine-rhodamine conjugate. Note the large bundles of microtubules which form a well-developed cyhyrigeal basket.
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Application
IHC-P analysis of human heart sections using alpha Tubulin antibody [DM1A] at a dilution of 1:500.
-
Application
WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH-3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK ａnd (9) MDCK lysates using alpha Tubulin antibody [DM1A] at a dilution of 1:500.
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Application
Whole cell extract of chicken gizzard was separated on SDS-PAGE ａnd blotted with Monoclonal Anti-α-Tubulin . The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate ａnd AEC substrate. Lane A: Antibody dilution 1:1,000 Lane B: Negative control (only secondary antibody)
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Application
Human skin fibroblasts stained with Monoclonal Anti-α-Tubulin, clone No. DM1A .
| Positive Control | Chicken fibroblasts |
|---|---|
| Application Notes | IF: Use at a dilution of 1/500, this is determined by indirect immunofluorescent labeling of cultured chicken fibroblasts. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 50 kDa. Optimal dilutions/concentrations should be determined by the end user.: More |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot ａnd store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Raw Ascites containing 15mM sodium azide |
原创作者：上海笃玛生物科技有限公司
