企业档案

  • 会员类型:免费会员
  • 工商认证: 【已认证】
  • 最后认证时间:
  • 法人:
  • 注册号:
  • 企业类型:生产商
  • 注册资金:人民币万

联系我们

联系人:杨燕燕

点击查看联系方式

技术文章

PPP2R4 [G4-D9] Catalog Number:OM627685

点击次数:25 发布时间:2024/9/24 14:02:10
 

Product Profile

Product Name PPP2R4 [G4-D9]
Antibody Type Primary Antibodies
Product description
n general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. PPP2R4 (protein phosphatase 2A activator, regulatory subunit 4), also known as PR53 or PTPA, is a 358 amino acid protein that is widely expressed and associates with the PP holoenzyme. Specifically, PPP2R4 functions to stimulate the ATP- and magnesium-dependent phosphotyrosyl phosphatase activity of the dimeric form of PP2A, thereby affecting the control of cell growth and division. Four isoforms of PPP2R4, designated alpha, beta, delta and epsilon, are expressed due to alternative splicing events.
More 
Immunogen
Recombinant protein

Key Feature

Clonality Monoclonal
Isotype IgG1
Host Species Mouse
Tested Applications

WBICCIHCFC

 
WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:100-1:500
FC:1:100-1:200
More 
Species Reactivity

Human

Concentration 2 mg/mL.

Target Information

Alternative Names
subunit B antibody KIAA0044 antibody MGC2184 antibody Phosphotyrosyl phosphatase activator antibody PP2A antibody PP2A phosphatase activator antibody PP2A subunit B antibody PP2A subunit B isoform PR53 antibody PP2A subunit B PR53 isoform antibody PPP2R4 antibody PR53 antibody PR53 isoform antibody Protein phosphatase 2
regulatory subunit B-prime antibody Protein phosphatase 2A activator regulatory subunit 4 antibody Protein phosphatase 2A regulatory subunit B' (PR 53) antibody PTPA antibody PTPA_HUMAN antibody Serine/threonine protein phosphatase 2A regulatory subunit B antibody Serine/threonine-protein phosphatase 2A activator antibody Serine/threonine-protein phosphatase 2A regulatory subunit 4 antibody Serine/threonine-protein phosphatase 2A regulatory subunit B antibody
More 
Molecular Weight(MW) 41 kDa
Cellular Localization Cylasm. Nucleus.

Database Links

SwissProt ID Q15257

Application

  • Application

    Fig1: Western blot analysis of PPP2R4 on human PPP2R4 recombinant protein using anti-PPP2R4 antibody at 1/1,000 dilution.

  • Application

    Fig2: ICC staining PPP2R4 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissue using anti-PPP2R4 antibody. Counter stained with hematoxylin.

  • Application

    Fig4: Immunohistochemical analysis of paraffin-embedded lung cancer tissue using anti-PPP2R4 antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Flow cytometric analysis of MCF-7 cells with PPP2R4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

Positive Control Human PPP2R4 recombinant protein, Hela, cervical cancer tissue, lung cancer tissue, MCF-7.
Application Notes
WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:100-1:500
FC:1:100-1:200
More 

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

原创作者:上海笃玛生物科技有限公司

相关产品

script>