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技术文章
PPP2R4 [G4-D9] Catalog Number:OM627685
Product Profile
Product Name | PPP2R4 [G4-D9] |
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Antibody Type | Primary Antibodies |
Product description |
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Immunogen | Recombinant protein |
Key Feature
Clonality | Monoclonal |
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Isotype | IgG1 |
Host Species | Mouse |
Tested Applications | WBICCIHCFC |
WB:1:500-1:1,000 More ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 | |
Species Reactivity | Human |
Concentration | 2 mg/mL. |
Target Information
Alternative Names | More |
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Molecular Weight(MW) | 41 kDa |
Cellular Localization | Cylasm. Nucleus. |
Database Links
SwissProt ID | Q15257 |
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Application
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Application
Fig1: Western blot analysis of PPP2R4 on human PPP2R4 recombinant protein using anti-PPP2R4 antibody at 1/1,000 dilution.
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Application
Fig2: ICC staining PPP2R4 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Application
Fig3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissue using anti-PPP2R4 antibody. Counter stained with hematoxylin.
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Application
Fig4: Immunohistochemical analysis of paraffin-embedded lung cancer tissue using anti-PPP2R4 antibody. Counter stained with hematoxylin.
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Application
Fig5: Flow cytometric analysis of MCF-7 cells with PPP2R4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Positive Control | Human PPP2R4 recombinant protein, Hela, cervical cancer tissue, lung cancer tissue, MCF-7. |
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Application Notes | WB:1:500-1:1,000 More ICC:1:100-1:500 IHC:1:100-1:500 FC:1:100-1:200 |
Additional Information
Form | Liquid |
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Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
原创作者:上海笃玛生物科技有限公司