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技术文章
PHB Antibody Catalog Number：OM287957
Product Profile
| Product Name | PHB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | This PHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the Central region of human PHB. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: | |
| Species Reactivity | Human |
| Concentration | 1 mg/ml |
Target Information
| Gene Symbol | PHB |
|---|---|
| Alternative Names | |
| Molecular Weight(MW) | 30 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Database Links
| Entrez Gene | 5245 |
|---|---|
| Protein Accession | P35232 |
Application
-
Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
-
Application
Confocal immunofluorescent analysis of PHB Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
-
Application
Western blot analysis of PHB Antibody in Hela cell line lysates (35ug/lane)
-
Application
Western blot analysis of PHB using rabbit polyclonal PHB Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHB gene.
-
Application
Formalin-fixed ａnd paraffin-embedded human hepatocarcinoma tissue reacted with PHB antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
| Application Notes | For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
原创作者：上海笃玛生物科技有限公司
