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技术文章
Anti-PHB1 antibody Catalog Number：OM642067
点击次数：38 发布时间：2024/9/26 14:35:12
Product Profile
| Product Name | Anti-PHB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinent Proteins |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHCWB |
| WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500 | |
| Species Reactivity | HumanRabbit |
| Concentration | 1 mg/ml |
| Purification | Protein A |
Target Information
| Gene Symbol | PHB |
|---|---|
| Gene Synonyms | |
| Gene Full Name | prohibitin |
| Gene Summary | This gene is evolutionarily conserved, ａnd its product is proposed to play a role in human cellular senescence ａnd tumor suppression. Antiproliferative activity is reported to be localized to the 3＇ UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] More |
| Molecular Weight(MW) | 30 kDa |
| Source | Mouse |
| Cellular Localization | Cell membrane, Cylasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus |
Application
-
WB
Western blot analysis using PHB1 mouse mAb against HT1080 (1), MCF-7 (2), NIH 3T3 (3), K562 (4) ａnd COS-7 (5) cell lysate.
-
IHC
Immunohistochemical analysis of paraffin-em
bedded prostate cancer tissues using PHB1 antibody with DAB staining. -
ICC/IF
Immunofluorescence analysis of PC-3 cells using PHB1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
| Application Notes | WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500 |
|---|
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
原创作者：上海笃玛生物科技有限公司
