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技术文章

MCM2 Antibody Catalog Number:OM284350

点击次数:29 发布时间:2024/9/27 14:31:02
 

Product Profile

Product Name MCM2 Antibody
Antibody Type Primary Antibodies
Product description
The protein encoded by MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.1) Yu, Z., et al., (2004) J. Mol. Biol. 340 (5), 1197-1206.
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Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM2.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

ELISAWB

 
MCM2 antibody can be used for detection of MCM2 by ELISA at 1:312500. MCM2 antibody can be used for detection of MCM2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Species Reactivity

Human

Concentration 1 mg/ml
Purification Protein A

Target Information

Gene Symbol MCM2
Alternative Names
MCM2
BM28
CCNL1
CDCL1
cdc19
D3S3194
MITOTIN
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Molecular Weight(MW) 99 kDa

Database Links

Entrez Gene 4171
Protein Accession NP_004517

Application

  • Application

    Antibody used in WB on mouse neural stem at 1:1000.

  • Application

    Antibody used in WB on Human Jurkat 1.25 ug/ml.

Positive Control 1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
MCM2 antibody can be used for detection of MCM2 by ELISA at 1:312500. MCM2 antibody can be used for detection of MCM2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:

Additional Information

Form Liquid
Storage Instructions For short periods of storage (days) store at 4˚C. For longer periods of storage, store MCM2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt

原创作者:上海笃玛生物科技有限公司

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