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技术文章
MCM2 antibody , N-term Catalog Number：OM262649
Product Profile
| Product Name | MCM2 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human MCM2. The exact sequence is proprietary. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. : | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (88%), Rat (88%) |
| Concentration | 1 mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
Target Information
| Gene Synonyms | More |
|---|---|
| Molecular Weight(MW) | 102 kDa (note) |
Application
-
Application
MCM2 antibody [N1N2], N-term immunoprecipitates MCM2 protein in IP experiments. IP samples: HCT-116 whole cell extract A. 40 μg HCT-116 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of MCM2 protein by 4 μg MCM2 antibody [N1N2], N-term 7.5 % SDS-PAGE The immunoprecipitated MCM2 protein was detected by MCM2 antibody [N1N2], N-term diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
-
Application
MCM2 antibody [N1N2], N-term detects MCM2 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM2 protein stained by MCM2 antibody [N1N2], N-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
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Application
Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE diluted at 1:1000
| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. : |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
原创作者：上海笃玛生物科技有限公司
