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技术文章
MCM2 Catalog Number：OM640904
Product Profile
| Product Name | MCM2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human MCM2 expressed in E. Coli. |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 More IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
Target Information
| Alternative Names | More |
|---|---|
| Molecular Weight(MW) | 125kDa |
Database Links
| Entrez Gene | 4171 |
|---|
Application
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Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
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Application
Figure 1: Western blot analysis using MCM2 mAb against human MCM2 (AA: 16-232) recombinant protein. (Expected MW is 50.4 kDa)
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Application
Figure 2: Western blot analysis using MCM2 mouse mAb against MCF-7 (1), Hela (2), Jurkat (3), K562 (4), HEK293 (5) ａnd HEPG2 (6) cell lysate.
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Application
Figure 3: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MCM2 mouse mAb with DAB staining.
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Application
Figure 4: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MCM2 mouse mAb with DAB staining.
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Application
Figure 5: Immunofluorescence analysis of Hela cells using MCM2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Application
Figure 6: Flow cytometric analysis of Jurkat cells using MCM2 mouse mAb (green) ａnd negative control (red).
| Application Notes | WB:1:250~1:1000 More IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
Additional Information
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
原创作者：上海笃玛生物科技有限公司
