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技术文章
HLA-G antibody Catalog Number：OM257160
点击次数：66 发布时间：2024/9/30 16:28:29
Product Profile
| Product Name | HLA-G antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant full length protein (Human). |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-G/9 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIHC-FWB |
| ELISA: Use at an assay dependent concentration. FACS: Use at an assay dependent concentration. IHC-Fr: Use at an assay dependent concentration. Is unsuitable for IHC-P. Optimal dilutions/concentrations should be determined by the end user.: | |
| Species Reactivity | HumanPrimate |
| Concentration | 1 mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
Target Information
| Gene Synonyms | More |
|---|---|
| Target | HLA-G |
| Tissue Specificity | Reacts with the native form of human HLA-G on the cell surface as well as with soluble HLA-G molecule in its beta 2-microglobulin associated form. Specificity was demonstrated with cells transfected with either full-length HLA-G ａnd HLA-G1 complementary DNA.This antibody does not compete with a tested panel of other monoclonal antibodies to HLA-G. |
| Cellular Localization | Cell Membrane |
Application
-
Application
Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC ( ) ａnd anti-human HLA-G (MEM-G/11) FITC .
-
Application
Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) ａnd protein G. HLA-G was detected by anti-human HLA-G (4H84) ａnd goat anti-mouse HRP in cell lysate (Lane 1) ａnd in the immunoprecipitate (Lane 2).
| Application Notes | ELISA: Use at an assay dependent concentration. FACS: Use at an assay dependent concentration. IHC-Fr: Use at an assay dependent concentration. Is unsuitable for IHC-P. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot ａnd store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
原创作者：上海笃玛生物科技有限公司
