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采用Applied Biosystems® TaqMan®基因表达预混液，您可以进行线性动态范围大于9 log的精确、可靠的实时PCR定量检测。TaqMan®基因表达预混液是一种经过优化的2倍混合物，其中含有进行低至一个拷贝数敏感度的靶标基因检测所需的所有成分（模板和引物除外）。其具有扩展的实验稳定性，可在自动液体处理系统上获得一致的结果，因而成为过夜乃至持续一周的高通量样品处理应用的理想选择。
TaqMan®基因表达预混液已经过基于TaqMan® 基因表达Assay和Applied Biosystems®实时荧光定量系统的验证，可以为一系列基于基因表达的实时qPCR应用提供精确的定量分析，其中包括：
罕见转录本检测
基因表达分析
病原体检测及病毒载量定量
两种靶标共扩增
基因家族成员的特异性检测
TaqMan® 基因表达预混液含有：AmpliTaq Gold® DNA聚合酶UP（超纯级），适于热启动活化，具有增强的细菌靶标基因的检测能力；dNTP和dTTP/dUTP混合物以及尿嘧啶DNA 糖激酶(UDG)，可以限度减少残余污染；基于ROX染料的被动内部参照，有助于在Applied Biosystems®实时PCR系统上进行更为精确定量检测。
规格
| Detection Method: | Primer-Probe Detection |
|---|---|
| Platform: | 7300 System, 7000 System, 7900HT System, StepOnePlus?, Standard Mode, StepOne?, Standard Mode, 7500 System |
| Reaction Speed: | Standard |
| PCR Method: | qPCR |
| Sample Type (General): | DNA |
| Polymerase: | AmpliTaq Gold® DNA Polymerase |
| Product Size: | 1 x 5 mL |
| Passive Reference Dye: | ROX (Pre-mixed) |
| Sample Type (Specific): | cDNA, DNA (Genomic) |
| Purity or Quality Grade: | UP (Ultra Pure) |
| Primer-Probe Compatibility: | TaqMan® |
内容及储存
1 bottle (5 ml) 2X TaqMan® Gene Expression Master Mix sufficient for 200 reactions. Store at 2°C to 8°C.
TaqMan® Gene Expression Master Mix contains AmpliTaq Gold® DNA Polymerase (Ultra Pure), Uracil-DNA glycosylase, dNTPs (with dUTP), ROX? Passive Reference, ａnd optimized buffer components.
TaqMan® Gene Expression Master Mix contains AmpliTaq Gold® DNA Polymerase (Ultra Pure), Uracil-DNA glycosylase, dNTPs (with dUTP), ROX? Passive Reference, ａnd optimized buffer components.
| 4369016 | TaqMan® Gene Expression Master Mix, 1-Pack (1 x 5 mL) | 500 rxn | 4705 |
| 4369514 | TaqMan® Gene Expression Master Mix, 2-Pack (2 x 5 mL) | 1000 rxn | 8820 |
| 4370048 | TaqMan® Gene Expression Master Mix, Mini-Pack (1 x 1mL) | 100 rxn | 1050 |
| 4440038 | TaqMan® Universal Master Mix II, with UNG 1-Pack (1 x 5 mL) | 200 rxn | 5340 |
| 4440040 | TaqMan® Universal Master Mix II, no UNG 1-Pack (1 x 5 mL) | 200 rxn | 4959 |
| 4440042 | TaqMan® Universal Master Mix II, with UNG Mini-Pack (1 x 1 mL) | 100 rxn | 1122 |
| 4440043 | TaqMan® Universal Master Mix II, no UNG Mini-Pack (1 x 1 mL) | 100 rxn | 1122 |
| 4440048 | TaqMan® Universal Master Mix II, no UNG 5-Pack (5 x 5mL) | 2500 rxn | 22050 |
| 4440045 | TaqMan® Universal Master Mix II, with UNG 5-Pack (5 x 5mL) | 2500 rxn | 23360 |
| 4444556 | TaqMan® Fast Advanced Master Mix (1 x 1 mL) | 100 rxn | 1170 |
| 4444557 | TaqMan® Fast Advanced Master Mix (1 × 5 mL) | 500 rxn | 5840 |
| 4444963 | TaqMan® Fast Advanced Master Mix (2 × 5 mL) | 1000 rxn | 11000 |
| 4391128 | TaqMan® PreAmp Master Mix | 40 rxn | 10510 |
| 4488593 | TaqMan® PreAmp Master Mix | 200 rxn | 43220 |
| 4371353 | TaqMan® Genotyping Master Mix, Mini-Pack (1mL) | 100 rxn | 1119 |
| 4371355 | TaqMan® Genotyping Master Mix, 1-Pack (1 x 10 mL) | 1000 rxn | 8680 |
| 4381656 | TaqMan® Genotyping Master Mix, 2-Pack (2 x 10 mL) | 2000 rxn | 15760 |
