描述

TaqMan® Fast Advanced Master Mix has been designed to match or exceed the performance of standard ｍaster mixes, delivering shorter run times (<40 minutes) with results equal to or better than what is achieved today. TaqMan® Fast Advanced Master Mix contains AmpliTaq® Fast DNA Polymerase, uracil-N glycosylase (UNG), dNTPs with dUTP, ROX™ dye (passive reference), ａnd optimized buffer components. It is supplied at a 2X concentration.

Features of the TaqMan® Fast Advanced Master Mix include:

• Best-in-class performance—superior sensitivity, accuracy, dynamic range, ａnd specificity compared to standard mixes in standard mode
• Engineered for enhanced benchtop stability—stable at room temperature for up to 72 hours in preassembled reactions
• Optimized for multiplexing—validated for duplexing with exogenous ａnd endogenous internal positive control assays
• Reduced run times on fast ａnd standard instrumentation—optimized for fast mode on fast instruments ａnd fast cycling conditions on standard instruments
• Seamlessly transitions into your experiments—validated with Applied Biosystems® TaqMan® Assays for Gene Expression ａnd microRNAs, ａnd TaqMan® Array Micro Fluidic Cards

Best-in-Class Performance
TaqMan® Fast Advanced Master Mix has been designed to provide performance equal to or better than the results you currently expect from your standard ｍaster mix. The ｍaster mix has been benchmarked against the leading suppliers＇ standard ａnd fast ｍaster mixes to help ensure that it succeeds in providing best-in-class sensitivity, accuracy, dynamic range, ａnd specificity. The impressive sensitivity of the mix showcased in Figure 1 compares the Ctvalues of TaqMan® Fast Advanced Master Mix ａnd TaqMan® Universal PCR Master Mix across a panel of gene expression assays.

Benchtop Stability for High-Throughput Handling ａnd Convenience
TaqMan® Fast Advanced Master Mix has been engineered to retain a high level of performance in preassembled reactions for up to 72 hours. The stability of this mix provides users of high-throughput, liquid handling systems the assurance that the results on the first plate will mimic those of the last plate. Figure 2 shows an assay that was run upon assembly (time 0) ａnd after 72 hours of incubation at 30°C, simulating the most extreme room temperature scenario.

For those with less extreme throughput needs, the enhanced stability of this ｍaster mix provides an overall added convenience to your workflow, as you are no longer constrained to immediately running your plates upon assembly.

Optimized for Multiplexing
We realize that confidence is paramount when it comes to your results. For added confidence in the results you see in every well, TaqMan® Fast Advanced Master Mix has been designed to help deliver accurate results for duplex reactions using an internal positive control (IPC). Figure 3 shows results for the experimental target β-actin (gene name ACTB), which was serially diluted ａnd amplified in single-target reactions ａnd duplex reactions.