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重组人胰岛素样生长因子结合蛋白-1齐一生物
点击次数：474 发布时间：2016/9/23
For research use only
Recombinant Human IGF-BP1
CATALOG #: 4717-10 10 µg
4717-25 25 µg
4717-100 100 µg
4717-1000 1 mg
LOT #: _____________________
SOURCE: NSO cells
PURITY: >98% by SDS-PAGE ａnd HPLC analyses
Endotoxin level is <0.1 ng per µg of IGF-BP1.
MOL. WEIGHT: 27.5 kDa
FORM: Sterile filtered ａnd lyophilized with no additives
RECONSTITUTION:
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml.
The solution can then be diluted into other aqueous buffers ａnd store at 4°
C for 1 week or –
20°
C for future use.
STORAGE CONDITIONS:
The lyophilized IGF-BP1 is best-stored desiccated below 0°
C. Reconstituted IGF-BP1 should
be stored at working aliquots at -20°
C.
DESCRIPTION:
Human IGF-BP1 is a 25.4 kDa cysteine-rich secreted protein expressed in liver, decidual, and
kidneys ａnd is the most abundant IGF-BP in amniotic fluid. Levels of IGF-BP1 in serum are
lowest after food. IGF-BP1 binds both IGF-I and IGF-II with equal affinity. Phosphorylated
IGF-BP1 hinders IGF actions, whereas nonphosphorylated IGF-BP1 is stimulatory.
Recombinant human IGF-BP1 is a single polypeptide consisting of 255 amino acid residues
with N-terminal His-tag..
BIOLOGICAL ACTIVITY:
The ED50 as determined by its ability to inhibit IGF-I induced proliferation of NUC-1 cells is
~100 ng/ml.
FOR RESEARCH USE ONLY! Not to be used on humans.
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