价格详细资料：试剂盒价格电议，齐一生物销售：021－6034 8496；181214 53965；173021 04490,齐一生物科技（上海）有限公司提供ELISA试剂盒受到了广大研究所.中科院科研单位一致肯定和认同。齐一生物大品牌保证，倾力为国内外科研院校实验室提供*优质产品。

【大鼠骨成型蛋白2(BMP-2)ELISA试剂盒厂家】注意事项

1. 当混合蛋白溶液时应尽量轻缓，避免起泡。

2. 洗涤过程非常重要，不充分的洗涤易造成假阳性。

3. 一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样。

4. 请每次测定的同时做标准曲线，做复孔。

5. 如标本中待测物质含量过高，请先稀释后再测定，计算时请*后乘以稀释倍数。

6. 在配制标准品、检测溶液工作液时，请以相应的稀释液配制，不能混淆。

7. 底物请避光保存。

8. 不要用其它生产厂家的试剂替换试剂盒中的试剂。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【大鼠骨成型蛋白2(BMP-2)ELISA试剂盒厂家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【大鼠骨成型蛋白2(BMP-2)ELISA试剂盒厂家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【大鼠骨成型蛋白2(BMP-2)ELISA试剂盒厂家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

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9238616  Buffer 20/30, Zent3          CNY                             

9238476  Compression ring caps, RapidPlate           CNY                             

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9238244  User Manual BR EZ1                  CNY                             

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9240659  Rebuild Kit, Pump, Jun-Air, AP          CNY                             

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9240648  Cable, Barcode, MS-3 CCD, AP196/98                CNY                             

9240645  External BCR, Metrologic, MS6720, AP            CNY                             

9240627  Fuse, 10amp, 5 X 20 mm, AP196/98                   CNY                             

9240620  Relay, DPST 24VCD, AP96                  CNY                             

9240595  MAC Valve, Manifold, Vacuum, AP98                CNY                              【大鼠骨成型蛋白2(BMP-2)ELISA试剂盒厂家】

9240592  Unload Valve, Vacuum Pump, AP196/98           CNY                             

9240565  Fan, Motor, 230V, Eppendorf AP196/98            CNY                             

9240555  Motor Cover, Rubber, Eppendorf, AP196/98              CNY                             

9240510  Cable Kit, PC104, AP196/98               CNY                             

9240501  PM Kit, AP98             CNY                             

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9239850  Adapter, XYP to LC200              CNY                             

9019943  Cable, P-axis ribbon, 8 ch. , CX1                 CNY                             

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9241208  QIAsymphony SP, Premium Agreement   "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: not applicable

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9241200  QIAxcel, Basic Agreement, no PM  "Repair service delivery send to Regional Repair Center

Service response time (repair) 7-10 days turnaround time

Periodic inspection/maintenance: 0

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

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9019468  PLATE GLASS LONG SET GS                CNY                             

9233375  Prssure retaining valve for Washer Mod.                  CNY                             

9237348  AdapterPlate, W-Drive, BR8 RD v2.0                CNY                             

9237329  Upgrade, BR & 3 ext. devices w. sched.  Upgrade BioRobot with three approved integration devices using scheduling software  CNY                              【大鼠骨成型蛋白2(BMP-2)ELISA试剂盒厂家】

9237168  Bottle Sheath Supply                 CNY                             

QY-x1657 615小鼠前胃癌瘤株Fc                                               

QY-x1658 小鼠乳腺癌细胞株EMT-6                                                   

QY-x1659 小鼠肾癌细胞株RuCa                                                 

QY-x1660 大鼠肉瘤细胞WK256                                                  

QY-x1661 小鼠乳腺癌细胞MA891                                                      

2QY-x1662        大鼠胰腺癌细胞株DSL-6A/C1                                                     

QY-x1663 小鼠骨髓瘤细胞NS1                                                   

QY-x1664 大鼠卵巢癌细胞株NUTU-19                                                       

QY-x1665 小鼠肾癌细胞Ranca                                                   

QY-x1666 小鼠胚胎成纤维细胞3T3-swiss albino                                                【大鼠骨成型蛋白2(BMP-2)ELISA试剂盒厂家】

QY-x1667 小鼠胚胎成纤维细胞3T3 clone A31                                                   

QY-x1668 小鼠胰腺腺泡细胞癌细胞MPC-83                                                     

QY-x1669 小鼠白血病细胞C3                                                      

QY-x1670 小鼠β胰岛素瘤细胞RIN-m5F                                                    

QY-x1671 小鼠自发高乳腺癌细胞TA2                                               

QY-x1672 小鼠淋巴瘤细胞Nb2－11                                                   

QY-x1673 小鼠骨髓瘤细胞NS-1                                                  

QY-x1674 小鼠肾癌细胞Kert-3                                                    

QY-x1675 HPVl6 E6、E7和ras基因共转化的C57BL／C(H-2b)小鼠肺上皮细胞TC-1                                                      

QY-x1676 长臂猿淋巴瘤MLA-144                                              

QY-x1677 细胞名称         细胞代号         ATCC号码        组织来源         培养基     ATCC描述/中科院细胞库描述      

QY-x1678 人胚肾细胞     293T         CRL-11268?     肾     DMEM＋10％FBS            

QY-x1679 人肾上皮细胞系     293            肾     DMEM＋10％FBS            

QY-x1680 人肾上皮细胞系     293H                  肾     DMEM＋10％FBS            

QY-x1681 人脐静脉内皮细胞         HUVEC               脐带静脉         ECM或             

QY-x1682 EGM-2                                                    【大鼠骨成型蛋白2(BMP-2)ELISA试剂盒厂家】

QY-x1683 人胚肺成纤维细胞         HLF  胚胎；肺         MEM α(+)+10%FBS                         

QY-x1684 人胚肺二倍体细胞         CCC-HPF-1         胚胎；肺         DMEM+ 10% FBS                       

QY-x1685 人微血管内皮细胞         MVEC                 微血管     DMEM+ 10%NBCS           

QY-x1686 人支气管平滑肌细胞     HBSMC                             RPMI 1640 + 10% NBCS           

QY-x1687 人肝癌细胞     Hep G2     HB-8065?          肝细胞癌         DMEM＋10％NBCS                  

QY-x1688 人肝癌细胞     BEL-7402          肝癌         RPMI 1640 + 10% NBCS           

QY-x1689 鼠肝癌细胞     H22  （悬浮）                   肝癌         RPMI 1640 + 10% NBCS