【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【大鼠乙酰胆碱转移酶(ChAT)ELISA试剂盒现货】样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【大鼠乙酰胆碱转移酶(ChAT)ELISA试剂盒现货】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【大鼠乙酰胆碱转移酶(ChAT)ELISA试剂盒现货】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【大鼠乙酰胆碱转移酶(ChAT)ELISA试剂盒现货】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

9238884  Stepper motor, turntable, BS96            CNY                           

9238866  BR MDx, Premium Agreement      The Premium Cover Agreement MDx provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day). CNY                           

9238857  Basic Cover Agreement Plato3, 1yr       The Basic Cover Agreement Plato3 provides full service coverage for the Plato3 platform. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.       CNY                           

9238838  #Twister I, Basic Agreement, no PM    The Basic Cover Agreement BR Twister I provides full service coverage for the BioRobot Twister I. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Cover Agreement, the response time is 5 working days.  CNY                           

9241220  QIAxcel, Installation      "The Installation, QIAxcel is an on-site service, conducted by a QIAGEN Service Specialist. During the installation, the QIAxcel hardware is set up according to the manufacturer’s instructions.

This installation covers the handling of the QIAxcel systems hardware ａnd familiarize with the basic instrument ａnd software functions of the BioCalculator software. All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements."  CNY                           

9241195  Cable, RS232, Belkin F2N209-06-T, 1.8m           CNY                           

9241186  Sensor, interlock2, with cable, QX              CNY                           

9238831  BR 8000, Basic Agreement    The Basic Cover Agreement 8000 provides full service coverage for the BioRobot 8000. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.       CNY                           

9238822  Rect. carrier for 1 tube 100 ml             CNY                           

9237194  Reservoir RapidPlate             CNY                           

9237171  Heater Block XYP         CNY                           

9233168  Mixer Board           CNY                           

9237154  Drive Mechanism XYP         CNY                           

9237110  Upgrade App., QIAamp 96 DNA Blood On-site application Upgrade for standard QIAGEN QIAamp 96 DNA Blood including kit    CNY                           

9237095  Training, HiLight On-site       On-site Training 1 day HiLight      CNY                            【大鼠乙酰胆碱转移酶(ChAT)ELISA试剂盒现货】

9237084  Installation, BR plus 3 integrations        CNY                           

9236414  Plate Stacker TW II              CNY                           

9232973  Level Detector Board P7-8 channels            CNY                           

9232954  LSW Board (Level sensor waste)         CNY                           

9232921  Comparator board 1.02 w/clot detection             CNY                           

9236337  Digital Board          CNY                           

9236155  Tool, calibration, syringe, RapidPlate           CNY                           

9235702  Cables, Shaker, RCS1           CNY                           

9235626  Lead Screw Nut, MP Holder LH, Shaker8000             CNY                           

9235297  Plunger tip, syringe 250µl             CNY                           

9235122  Fuse T1A, v1.00           CNY                           

9235121  Fuse T2,5A, v1.00        CNY                           

9235119  Fuse F3.15A, v1.00              CNY                           

9238667  Front panel            CNY                           

9238646  Diode BY 500-600, 600 V 5 A              CNY                           

9238621  Power supply, motorized lid locks        CNY                            【大鼠乙酰胆碱转移酶(ChAT)ELISA试剂盒现货】

QY-x2218       BABL/C1 BABL/C小鼠13-14天胚胎细胞                                    

QY-x2219       BABL/C2 BABL/C小鼠胚胎细胞                                    

QY-x2220       BABL/3T3 BABL/C小鼠胚胎成纤维细胞                                     

QY-x2221       BALL-1 人B淋巴细胞急性白血病细胞                                       

QY-x2222       Bcap-37 人乳腺癌细胞                                   

QY-x2223       BHK 叙利亚仓鼠肾细胞                                        

QY-x2224       BHK-21 叙利亚仓鼠肾细胞                                   

QY-x2225       BIU-87 人浅表性膀胱癌细胞                                        

QY-x2226       BRL-3A 大鼠肝细胞                                        【大鼠乙酰胆碱转移酶(ChAT)ELISA试剂盒现货】

QY-x2227       BT-325 人脑多形性胶质母细胞瘤细胞                                       

QY-x2228       BV-2 小鼠小脑胶质瘤细胞                                    

QY-x2229       BxPC3 人原位胰腺癌细胞                                     

QY-x2230       C127 小鼠乳腺癌细胞                                   

QY-x2231       C6/36 白纹伊蚊细胞                                      

QY-x2232       C6-121 大鼠神经胶质瘤细胞                                       

QY-x2233       C6-1752 大鼠神经胶质瘤细胞                                      

QY-x2234       C6-64 大鼠神经胶质瘤细胞                                         

QY-x2235       C6-70 大鼠神经胶质瘤细胞                                         

QY-x2236       C6-D2B 大鼠神经胶质瘤细胞                                      

QY-x2237       C8166-CD4 人T细胞性白血病细胞                                    

QY-x2238       CA 人肝癌细胞                                       

QY-x2239       Caco-2 人结肠腺癌细胞                                        

QY-x2240       CAM191 EBV-转化人淋巴细胞                                      

QY-x2241       CAM200 EBV-转化人淋巴细胞                                      

QY-x2242       CatK2 家猫肾上皮细胞                                          【大鼠乙酰胆碱转移酶(ChAT)ELISA试剂盒现货】

QY-x2243       CatL1 家猫肺成纤维样细胞                                         

QY-x2244       CatL2 家猫肺成纤维样细胞                                         

QY-x2245       CatS2 家猫皮肤成纤维样细胞                                      

QY-x2246       CFPAC-1 人胰腺癌细胞                                        

QY-x2247       CHL 中国仓鼠肺细胞                                     

QY-x2248       CHL-Don 中国仓鼠肺成纤维样细胞                                    

QY-x2249       CHO 中国仓鼠卵巢细胞                                       

QY-x2250       CHO/dhfr- 中国仓鼠卵巢细胞,