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人α乳清蛋白(α-La)ELISA试剂盒厂家
点击次数：17发布时间：2016/8/17 16:48:23
更新日期：2016/8/17 16:48:23
所 在 地：中国大陆
产品型号：
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详细内容
ELISA 检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。齐一生物销售：021－6034 8496；181214 53965；173021 04490
注意事项：
◆ 每个标本量收集体积＝ 100ul × 检测种类，如要做复孔，标本量收集体积＝ 100ul × 检测种类×2 。
◆ 对于作某一个具体指标来说，先用一系列稀释度作一批标本测定，得出对实验有意义的一个稀释度（即测定剂量反应曲线），然后用一个*适稀释度测定即可。
◆ 收集标本前必须清楚要检测的成份是否足够稳定。对收集后当天进行检测的标本，储存在4℃备用，如有特殊原因需要周期收集标本，将标本及时分装后放在-20℃ 或-70℃ 条件下保存。避免反复冻融。标本 2 -8 ℃ 可保存48 小时.-20℃ 可保存1个月。 -70 度可保存6个月。部分激素类标本需添加抑肽酶。
◆ 血清标本采集时，应注意避免溶血，红细胞溶解时会释放出具有过氧化物酶活性的物质，以 HRP为标记的 ELISA 测定中，溶血标本可能会增加非特异性显色。
【人α乳清蛋白(α-La)ELISA试剂盒厂家】◆ 为了保证尿液检测结果的准确性，必须正确收集尿液标本和保存。盛尿容器要清洁干燥。使用一次性的容器（如塑料尿杯），避免因用药并清洗不干净而造成的污染，影响检测结果。尿液标本必须新鲜，留取后，应及时检测或保存，以免细菌繁殖。因在室温中久置后（尤其是夏季）尿中磷酸盐等可析出结晶而干扰检测。
◆ 标本宜在新鲜时检测。如有细菌污染，菌体中可能含有内源性 HRP，也会产生假阳性反应。保存过久可发生聚合在 ELISA中可使本底加深。
◆ 冻结标本融解后，蛋白质局部浓缩，分布不均，应充分混匀宜轻缓，避免气泡，可上下颠倒混和，不要在混匀器上强烈振荡。
◆ 混浊或有沉淀的标本应先离心或过滤，澄清后再检测。
◆ 反复冻融会使蛋白效价降低，所以待测标本如需保存作多次检测，宜少量分装冰存。也可加入适当防腐剂。
◆ 如果您在实验中遇到任何特殊问题，敬请咨询 021-60348496标本类型
ELISA常见的标本一般包括：
◆ 液体类标本：包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清等。
◆ 培养细胞
◆ 组织标本
这些标本收集的时间、方法和保存都有一定的要求。
一般说来，在 5 天内测定的标本可放置于 4℃，超过一周测定的需低温冻存。
对收集后当天进行检测的标本，储存在 4 ℃ 备用，如有特殊原因需要周期收集标本， 将标本及时分装后放在 -20 ℃ 或 -70 ℃ 条件下保存。避免反复冻融。 标本 2 -8 ℃ 可保存 48 小时，-20 ℃ 可保存 1 个月。-70 度可保存 6 个月。部分标本也可加入适当防腐剂，激素类标本需添加抑肽酶。标本的处理 可用作 ELISA 测定的标本十分广泛，体液（如血清）、分泌物（唾液）和排泄物（如尿液）、细胞培养上清、细胞、组织等均可作标本以测定其中某种成份。有些标本可直接进行测定，有些则需经预处理。
◆ 血清：
室温血液自然凝固 10-20 分钟后，离心 20 分钟左右（ 2000-3000 转 / 分）。收集上清。如有沉淀形成，应再次离心。
◆ 血浆：
应根据试剂盒的要求选择 EDTA 、柠檬酸钠或肝素作为抗凝剂，加入 10 ％（ v/v ）抗凝剂 ( 0.1M 柠檬酸钠或 1% heparin 或 2.0%EDTA.Na2) 混合 10-20 分钟后，离心 20 分钟左右（ 2000-3000 转 / 分）。仔细收集上清如有沉淀形 成，应再次离心。
【人α乳清蛋白(α-La)ELISA试剂盒厂家】◆ 尿液、胸腹水、脑脊液：
用无菌管收集。离心 20 分钟左右（ 2000-3000 转 / 分）。仔细收集上清。如有沉淀形成，应再次离心。
◆ 细胞培养上清：
检测分泌性的成份时，用无菌管收集。离心 20 分钟左右（ 2000-3000 转 / 分）。仔细收集上清。检测细胞内的 成份时，用 PBS （ PH7.2-7.4 ）稀释细胞悬液，细胞浓度达到 100 万 /ml 左右。通过反复冻融，以使细胞破坏并放出细胞内成份。离心 20 分钟左右（ 2000-3000 转 / 分）。仔细收集上清。保存过程中如有沉淀形成，应再次离心。
◆ 细胞：
检测细胞的成份时，对于贴壁细胞，去除培养液，用PBS、生理盐水或无血清培养液洗一遍。加入适量裂解液。用枪吹打数下，使裂解液和细胞充分接触。通常裂解液接触细胞10 秒后，细胞就会被裂解。对于悬浮细胞，离心收集细胞，用PBS、生理盐水或无血清培养液洗一遍.加入适量裂解液, 用枪吹打把细胞吹散。用手指轻弹以充分裂解细胞。充分裂解后应没有明显的细胞沉淀。如果细胞量较多，必需分装然后再裂解。充分裂解后，10000-14000g离心3-5 分钟，取上
清，即可进行后续操作。
【人α乳清蛋白(α-La)ELISA试剂盒厂家】◆ 组织标本：
切割标本后，称取重量。加入一定量的 PBS或组织蛋白萃取试剂，缓冲液中可加入蛋白酶抑制剂。用手工或匀浆器将标本匀浆充分。离心 20 分钟左右（ 2000-3000 转 / 分）。仔细收集上清置于 -20 度或 - 70 度保存，如有必要，可以将样品浓缩干燥。分装后一份待检测，其余冷冻备用。齐一生物销售：021－6034 8496；181214 53965；173021 04490
9240651 Internal BCR, Keyence, BL-180, AP96/196 CNY
9240626 Fuse, 8amp, 5 X 20 mm, AP196/98 CNY
9240623 Circuit Breaker, 2A, AP196/98 CNY
9240591 3-Way Mac Valve, AP196/96 CNY
9240574 Micro-Lynx w\speedbaord, AP98 CNY
9240572 Rotor w/Buckets, Sorvall, AP96 CNY
9240552 Freq. Conv., PCB, Eppendorf, AP196/98 CNY
9240531 Cable, Motor, Axis #7, Pump U/D, AP98 CNY 【人α乳清蛋白(α-La)ELISA试剂盒厂家】
9240564 Fan, Condenser, 230V,Eppendorf, AP196/98 CNY
9240553 Cable, PC-104 to Eppendorf, AP196/98 CNY
9240548 Motor Cap Repair, Eppendorf, AP196/98 CNY
9240515 I/O PCB, Main Board, AP196/98 CNY
9240503 PM Kit, AP196 CNY
9239958 BR M48, PM Service "Service response time (repair): not applicable
Periodic inspection/maintenance: yes
Inspection/maintenance delivery: onsite
Cost coverage for Repair Parts : not included
Cost coverage for labor: yes, full coverage
Cost coverage for travel: yes, full coverage
Replacement system (Loaner) provision: not applicable
Transportation cost coverage: yes, full coverage" CNY
9239853 Foot, complete, v2.0 (4) CNY
9239572 BR Universal, Full Agreement The Full Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days). CNY
9239563 Assy,Kit,Retrofit,HoriCompressorMnt CNY
9020033 LED UV (325), RGQ CNY
9020023 L 26_7 Firmware, DML2 CNY
9019975 PCB, UV light assembly, Cxx CNY
9238858 Basic Cover Agreement Plato7, 1yr The Basic Cover Agreement Plato7 provides full service coverage for the Plato 7 platform. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days. CNY
9241233 User Manual, QIAxcel FA 01/08 (WW) CNY
9241227 ZLV, Handscanner, Qsym CNY
9241180 Motor, Z, QX CNY 【人α乳清蛋白(α-La)ELISA试剂盒厂家】
9019473 Loading guide, 52 well sharkstooth, GSC CNY
9019462 Switch, micro, HS CNY
9019457 Fans (2), 5V, 30mm, HS CNY
9237172 Cable USB CNY
9233169 Board, YZ-CPU, BR8000 CNY
9237163 Bezel LiquiChip CNY
9237162 Shipping Plug XYP CNY
ULBC plates 超低结合培养板（6孔） Ultra-Low Binding Culture Plate (6-well) "6-well, 4 plates
" 【人α乳清蛋白(α-La)ELISA试剂盒厂家】
ULBC plate 超低结合培养板（24孔） Ultra-Low Binding Culture Plate (24-well) "24-well, 1 plate
"
ULBC plates 超低结合培养板（24孔） Ultra-Low Binding Culture Plate (24-well) "24-well, 4 plates
"
rhbFGF 重组人碱性成纤维细胞生长因子－－碱性 Recombinant Human Fibroblast Growth Factor-basic 10µg
rhEGF 重组人表皮生长因子 "Recombinant Human Epidermal Growth Factor
" 100µg
rhBMP-2 重组人骨形成蛋白-2 "Recombinant Human Bone Morphogenetic Protein-2
" 2µg
rhBMP-4 重组人骨形成蛋白-4 "Recombinant Human Bone Morphogenetic Protein-4
" 2µg
rhBMP-7 重组人骨形成蛋白-7 "Recombinant Human Bone Morphogenetic Protein-7
" 2µg
rhNOGGIN 重组人NOGGIN蛋白 "Recombinant Human NOGGIN
" 5µg
HAMSC 人羊膜间充质基质细胞 Human Amniotic Mesenchymal Stromal Cells 5 x 10^5 cells/vial
HCMSC 人绒毛膜间充质基质细胞 Human Chorionic Mesenchymal Stromal Cells 5 x 10^5 cells/vial
HMSC-bm 人间充质干细胞－－骨髓 Human Mesenchymal Stem Cells-bone marrow 5 x 10^5 cells/vial
HMSC-ad 人间充质干细胞－－脂肪 Human Mesenchymal Stem Cells-adipose 5 x 10^5 cells/vial
HMSC-hp 人间充质干细胞－－肝脏 Human Mesenchymal Stem Cells-hepatic 5 x 10^5 cells/vial
HUMSC 人脐带间充质干细胞 Human Umbilical Mesenchymal Stem Cells 5 x 10^5 cells/vial
HPMSC 人肺间充质干细胞 Human Pulmonary Mesenchymal Stem Cells 5 x 10^5 cells/vial
HVMSC 人脊椎间充质干细胞 Human Vertebral Mesenchymal Stem Cells 5 x 10^5 cells/vial 【人α乳清蛋白(α-La)ELISA试剂盒厂家】
MSCM 间充质干细胞培养基 Mesenchymal Stem Cell Medium 500 ml
MODM-500 间充质干细胞-成骨细胞分化培养基 Mesenchymal Stem Cell Oseogenic Differentiation Medium 500 ml
MODM-250 Mesenchymal Stem Cell Oseogenic Differentiation Medium 250 ml
MODM-b 间充质干细胞-成骨细胞分化培养基-基础 Mesenchymal Stem Cell Osteogenic Differentiation Medium-basal 500ml
MODM-b-prf 间充质干细胞-成骨细胞分化培养基-无酚红 Mesenchymal Stem Cell Osteogenic Differentiation Medium-basal-phenol red free 500ml
MADM-500 间充质干细胞-脂肪细胞分化培养基 Mesenchymal Stem Cell Adipogenic Differentiation Medium 500 ml
MADM-250 Mesenchymal Stem Cell Adipogenic Differentiation Medium 250 ml
MADM-b 间充质干细胞-脂肪细胞分化培养基-基础 Mesenchymal Stem Cell Adipogenic Differentiation Medium-basal 500 ml
MADM-b-prf 间充质干细胞-脂肪细胞分化培养基-无酚红 Mesenchymal Stem Cell Adipogenic Differentiation Medium-basal-phenol red free 500ml
MCDM 间充质干细胞-软骨分化培养基 Mesenchymal Stem Cell Chondrogenic Differentiation Medium 500ml
Ared 茜素红S染色试剂盒 Alizarin Red S Staining Kit 100 ml
Ored 油红O染色试剂盒 Oil Red O Staining Kit 100 ml
PCR 人间质干细胞成脂肪细胞检测试剂盒 Human Mesenchymal Stem Cell Adipogenesis Detection Kit 50 reactions
HMSC-C PCR 人间充质干细胞软骨形成检测试剂盒 Human Mesenchymal Stem Cell Chondrogenesis Detection Kit 50 reactions
SafraninO 番红O染色试剂盒 Safranin O Staining Kit 100 reactions
ABlue 阿利新蓝染色试剂盒 Alcian Blue Staining Kit 100 reactions 【人α乳清蛋白(α-La)ELISA试剂盒厂家】
s�ci�*�)ily:"Times New Roman";Times New Roman"＇>人真皮成纤维细胞-胎儿C处理 Human Dermal Fibroblasts-Fetal-Mitomycin C treated 1 x 10^6 cells/vial
MEF-cm 小鼠胚胎成纤维细胞培养基 Mouse Embryonic Fibroblasts-Conditioned Medium 100 ml
bFGF-std MEF-cm 碱性成纤维细胞生长因子刺激的小鼠胚胎成纤维细胞条件培养基 bFGF-Stimulated Mouse Embryonic Fibroblasts Conditioned Medium 100ml
ULBC plates 干细胞超低结合培养板 Ultra-Low Binding Culture Plate 6-well, 1 plate
l�K���*�)si-font-family:"Times New Roman";color:black;
5. Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6. The substrate evade the light preservation.
7. Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8. All samples, washing buffer ａnd each kind of reject should according to infective material process.
9. Do not mix reagents with those from other lots.
Calculate：
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
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Storage ａnd validity
1．Storage： 2-8℃.
2．validity： six months.
9237124 Upgrade App., MinElute 96 Take advantage of our instrumentation ａnd molecular biology expertise to install MinElute 96 on your BioRobot System. The onsite protocol development visit includes all travel ａnd labor for the visit to install the standard QIAGEN MinElute 96 protocol, plus a MinElute 96 BioRobot Kit (4-plate) Cat # 28051 CNY
9237099 Upgrade BR configuration, on site CNY
9236424 Motor, rotary, TWI CNY
9232970 YZ 96 Connector Board 2 (Arm), E10103 CNY
9236409 Plate Stacker TW I, microplate CNY
9236357 Sample Holder Large, 1.5ml CNY 【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】
9232911 Board, YZ driver CNY
9236336 Analog Board CNY
9236158 Pot. , syr. or vert. , kit, RapidPlate CNY
9236149 Locator Plate, 96 well,pos 4-5,BR RP CNY
9236114 O-Ring, Coupling, RoboVac, BR8 RD v4.2 CNY
9236107 Flange Tool CNY
9230680 Probe, 0.4 mm, BR8000 v1.01 CNY
9230478 Rack f. Diamend Gel Cards (holds 6cards) CNY
9230473 Set, Cables, PCB (X-main) to shaker CNY
9235646 Connector, Board, HS shaker, BR8000 v1.0 CNY
9235255 T-piece, HSPS tubing, connector CNY
9235190 Motor, clamp, shaker plate, BR8000 CNY
9230060 Rack for reagents (12 x diameter 21mm) CNY
9238673 Hinge, imbalance system CNY
9238653 Power Board, temp. switch - heat sink CNY
9238635 Hook identification compl. CNY 【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】
9242028 RGQ, Repair Fee "Repair service delivery send to Regional Repair Center
Service response time (repair) 7-10 days turnaround time
Periodic inspection/maintenance: 0
Cost coverage for Repair Parts : yes, full coverage
Cost coverage for labor: yes, full coverage
Cost coverage for travel: yes, full coverage
Replacement system (Loaner) provision: not applicable
Transportation cost coverage: yes, full coverage" CNY
9241821 Pyro Q96 MDa, Basic Agreement "Repair service delivery: onsite
Service response time (repair): 5 business days
Periodic inspection/maintenance: yes, 1/agreement year
Inspection/maintenance delivery: onsite
Cost coverage for Repair Parts: yes, included
Cost coverage for labor: yes, included
Cost coverage for travel: yes, included
Replacement system (Loaner) provision: not included
Transportation cost coverage: not applicable" CNY
9241786 QIAgility Demopool Box Light Version CNY
9240976 PCB, display, sample tracking, Qsym CNY
9240968 Barcode camera, sample tracking, Qsym CNY
9240816 #Full Agreement, EZ1 DSP, 1YR CNY
9240680 Spill Tray, Shaker,Modified, AP CNY
9240790 Update,Waste Bottle Sensor, AP196/98 CNY
6000-5014 Lumicheck Plate Service Package, DML2 CNY
TGS 滋养层细胞生长因子 Trophoblast Growth Supplement 5 ml
PAdDS 前脂肪细胞分化生长因子 Preadipocyte Differentiation Supplement
PAGS 前脂肪细胞生长因子 Preadipocyte Growth Supplement 5 ml 【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】
AdGS 脂肪细胞生长因子 Adipocyte Growth Supplement 5 ml
OEpiCGS 卵巢上皮细胞生长因子 Ovarian Epithelial Cell Growth Supplement 5 ml
MODS 间充质干细胞成骨分化生长因子 Mesenchymal Stem Cell Osteogenic Differentiation supplement 5 ml
MADS 间充质干细胞脂肪分化生长因子 Mesenchymal Stem Cell Adipogenic Differentiation supplement 5 ml
MSCGS 间充质干细胞生长因子 Mesenchymal Stem Cell Growth Supplement 5 ml
MSCGS-sf 间充质干细胞生长因子 Mesenchymal Stem Cell Growth Supplement-serum free 5 ml
MSCGS-acf 间充质干细胞生长因子-无动物组分 Mesenchymal Stem Cell Growth Supplement-animal component free 5 ml
MCDS 间充质干细胞软骨分化生长因子 Mesenchymal Stem Cell Chondrogenic Differentiation Supplement 5 ml
MEpiCGS 乳腺上皮细胞生长因子 Mammary Epithelial Cell Growth Supplement 5 ml
FBS 胎牛血清 Fetal Bovine Serum, Human Cell Tested 10 ml
FBS 胎牛血清 Fetal Bovine Serum, Human Cell Tested 25 ml
T/E 胰酶/EDTA消化液 Trypsin/EDTA solution 100 ml
TNS 胰酶中和液 Trypsin Neutralization Solution 100 ml
TNS-sf 胰酶中和液-无血清 TNS-sf (Trypsin Neutralization Solution-serum free) 100 ml
ECDS 非酶细胞裂解液 Enzyme-Free Cell Dissociation Solution 100 ml
CFM 细胞冻存液 Cell Freezing Medium 50 ml 【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】
SF-CFM 无血清细胞冻存液 Serum-Free Cell Freezing Medium 50 ml
StemCryo 人胚胎干细胞冻存液 Human Pluripotent Stem Cell Cryopreservation Medium 50ml
StemCryo 人胚胎干细胞冻存液 Human Pluripotent Stem Cell Cryopreservation Medium, 5 x 10mL
TB 台盼蓝 Trypan Blue, 0.4% 50 ml
DPBS 磷酸盐缓冲液 Dulbecco’s Phosphate-Buffered Saline 500 ml
HBSS 平衡盐溶液 Hank＇s Balanced Salt Solution 500 ml
PLL 多聚赖氨酸 Poly-L-Lysine 1 mg/ml 1 ml
PLL 多聚赖氨酸 Poly-L-Lysine 10 mg/ml 1 ml
GSN 0.2％的明胶溶液 0.2% Gelatin Solution 100ml
FBS 胎牛血清 Fetal Bovine Serum, Human Cell Tested 500 ml
P/S 青霉素/链霉素溶液 Penicillin/Streptomycin Solution 5 ml
P/S 青霉素/链霉素溶液 Penicillin/Streptomycin Solution 100 ml
AMS 抗真菌溶液 Antimycotic Solution 50 ml
ABAMS 抗菌/抗霉菌溶液 Antibiotic/Antimycotic Solution 50 ml
