1.购买我公司任意ELISA试剂盒指标享受免费代测，并提供技术指导服务！

二、实验材料与试剂配制：

1. 仪器与材料：酶标仪（使用前预热30分钟），微量加液器、吸头、蒸馏水或去离子水，滤纸。

2. 缓冲液使用：加5ul  的缓冲液于50ul  的样品中，如果样品量不够或者不确定，缓冲液和样品的混合比例不要小于1:10即可混匀，静置1小时备用（如果标本是血清或者血浆，此步骤忽略）。

3. 洗液的配制：按1:100的比例配制洗液备用。

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人合胞病毒(RSV)IGMELISA试剂盒厂家】三. 仔细阅读说明书

1.检查试剂盒标签上的有效期。如果超过有效期，请勿使用。

2. 按说明书确定所有试剂齐全（数量、体积）

3. 标本制备要规范，每份标本体积要按2-3个复孔以上的量制备（贮存），尽量分装做备份。

4.短期内无法实验者，注意低温保存

5.准备好所有实验额外所需物品，（比如移液器，试管，清洗器，酶标仪）

6. 按说明书将所用试剂平衡至室温，根据检测标本数量确定所需试剂的量

7.检查试剂盒内每种试剂的贮存条件，保证所有试剂均按照试剂盒内说明书推荐的条件存储。 【人合胞病毒(RSV)IGMELISA试剂盒厂家】

8.检查不稳定或者变质的试剂溶液（比如沉淀或变色）。有些试剂，比如标准品稀释液或者检测稀释液，可能在设计时就含有沉淀物。所有试剂在滴入酶标板之前，必需充分混匀。而由于沉淀物的特性，在加入酶标板之前，必需不停搅拌。

9.保证充分的孵育时间和温度。

10.切勿使用不同生产批号的试剂取代现有试剂或混合现有试剂。

11.在混合或溶解蛋白溶液时，避免泡沫产生。

12.在实验开始之前，安排好实验流程。在实验开始之前，清洁工作台。

13.若有问题，应与我公司或代理商的技术支持联系

【人合胞病毒(RSV)IGMELISA试剂盒厂家】四、客户须知

齐一生物销售：021－6034 8496；181214 53965；173021 04490

1.在收集标本前都必须有一个完整的计划，必须清楚要检测的成份是否足够稳定。我们提倡新鲜标本尽早检测，对收集后当天就进行检测的标本，及时储存在4℃备用，如有特殊原因需要周期收集标本，请造模取材后，将标本及时分装后放在-20℃或-70℃条件下保存。因冰室与室温存在一定温差，蛋白极易降解，直接影响实验质量，所以避免反复冻融。部分标本也可加入适当防腐剂，激素类标本需添加抑肽酶。 

2.试剂盒代测标本的客户取材前须向我司销售人员索要说明书，具体操作注意事项请与我司技术人员沟通。

3.液体类标本：标本必须为液体，不含沉淀。包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等。

4.购买前请提前告诉我们：您样本的种属、样本数量、待测指标及其它特殊要求。

【人合胞病毒(RSV)IGMELISA试剂盒厂家】五、代测数据详细内容

1、ELISA标准曲线；

2、实验具体详细步骤；

3、完整的实验报告（试剂耗材，实验方法，实验结果及结果说明）；

4、实验进行阶段，我公司客服人员会及时向您汇报实验进程。

9238888  PCB, Main-01, BS96                   CNY                             

9238886  Cable, HM, BS96               CNY                              【人合胞病毒(RSV)IGMELISA试剂盒厂家】

9238854  #BR M96, Basic Agreement    The Basic Cover Agreement M96 provides full service coverage for the BioRobot M96. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238842  RS232 box, non-online fill, RapidPlate               CNY                             

9241215  Full Agreement Depot Repair, Shaker, 1yr       "The Full Agreement Depot Repair, Shaker provides full service coverage for the plate shaker used in the digene HPV application. The service is conducted as an instrument exchange service.

All shipping, labor ａnd repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."          CNY                             

9238833  #BR 9604, Basic Agreement    The Basic Cover Agreement 9604, 1 PM provides full service coverage for the BioRobot 9604. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9238832  BR 9600, Basic Agreement      The Basic Cover Agreement 9600 provides full service coverage for the BioRobot 9600. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238819  Rect. carrier for 5 glass tubes 25ml                   CNY                             

9237527  Software LiquiChip Workstation IS V2.2.          CNY                             

9237380  Installation, BR MDx & 2 react. setup     Installation ａnd training, including two customized reaction setup protocols         CNY                             

9237379  Installation, BR MDx & 1 react. setup     Installation ａnd training, including one customized reaction setup protocol CNY                             

9237378  BR MDx, Installation        The Installation, BR MDx facilitates the quick implementation of the BioRobot MDx into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the BioRobot MDx instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.         CNY                             

9237175  Guard Fan, Platic XYP                CNY                             

9233209  Tubing, 3/2,                CNY                             

9233155  Power Cord, European              CNY                             

9236418  Board, CPU, TWI               CNY                             

9236345  Red LSR             CNY                             

9236166  Safety shield, RapidPlate          CNY                              【人合胞病毒(RSV)IGMELISA试剂盒厂家】

9230863  Anpressplatte Rose-80              CNY                             

9235538  Teaching Tool            CNY                             

9230282  Cable, RS323, robot to syringe pump, 225                CNY                             

5050-2002        hc2 Software V2 Package                  CNY                             

BS96RNA BioSprint 96 to RNA (384) - UDP               CNY                             

9240730  Tubing, 1/4" OD, Red, AP          CNY                             

9240713  Poly Muffler Kit, AP96/196                 CNY                             

9240690  Bearings, Lead Screws, AP                CNY                             

9243654  Comprehen Valid., Other Quant. Sys.      """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   For planning purposes ａnd to ensure a smooth process, the Customer is expected to have all materials on-hand ａnd inventoried one (1) week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """     CNY                             

6000-5013        LumiCheck Plate User Package                  CNY                             

288103     bactotype Mycoplasma Mg/Ms PCR Kit (24)            CNY                             

1048147  QIAamp Viral RNA Mini Accessory Set              CNY                             

270043     cattletype BHV1 gB Ab (5)                CNY                             

QT-00005 福氏志贺氏菌(Shigellaflexneri)ATCC12022    冻干粉                                         

QT-00006 表皮葡萄球菌ATCC12228      冻干粉                                         

QT-00007 败毒梭菌ClostridiumsepticumATCC12464     冻干粉                                         

QT-00008 阴沟肠杆菌ATCC13047 冻干粉                                         

QT-00009 产气荚膜梭菌ATCC13124      冻干粉                                         

QT-00010 荧光假单胞菌ATCC13525      冻干粉                                         

QT-00011 婴儿双歧杆菌ATCC15697      冻干粉                                          【人合胞病毒(RSV)IGMELISA试剂盒厂家】

QT-00012 短双歧杆菌ATCC15700 冻干粉                                         

QT-00013 青春双歧杆菌ATCC15703      冻干粉                                         

QT-00014 黑曲霉ATCC16404 冻干粉                                         

QT-00015 恶臭假单胞菌ATCC17485      冻干粉                                         

QT-00016 单增李斯特氏菌ATCC19111 冻干粉                                         

QT-00018 溶组织梭菌ClostridiumhistolyticumATCC19401    冻干粉                                         

QT-00020 生孢梭菌ATCC19404      冻干粉                                         

QT-00022 破伤风杆菌/梭菌ClostridiumtetaniATCC19406     冻干粉                                         

QT-00024 化脓性链球菌ATCC19615      冻干粉                                         

QT-00025 黄杆菌属ATCC21047      冻干粉                                         

QT-00026 近平滑念珠菌ATCC22019      冻干粉                                         

QT-00027 变异链球菌ATCC25175 冻干粉                                         

QT-00028 脆弱拟杆菌ATCC25285 冻干粉                                         

QT-00029 洋葱假单胞菌ATCC25416      冻干粉                                         

QT-00030 大肠埃希氏菌ATCC25922      冻干粉                                         

QT-00032 金黄色葡萄球菌ATCC25923 冻干粉                                          【人合胞病毒(RSV)IGMELISA试剂盒厂家】

QT-00034 宋内氏志贺氏菌(Sh.sonnei)ATCC25931 冻干粉                                         

QT-00035 金黄色葡萄球菌ATCC27217 冻干粉                                         

QT-00037 创伤弧菌ATCC27562      冻干粉                                         

QT-00038 铜绿假单胞杆菌（绿脓杆菌）ATCC27853  冻干粉                                         

QT-00040 粪肠球菌ATCC29212      冻干粉                                         

QT-00041 金黄色葡萄球菌ATCC29213 冻干粉                                         

QT-00047 两歧双歧杆菌ATCC29521      冻干粉                                         

QT-00049 多形拟杆菌ATCC29741 冻干粉                                         

QT-00051 粪肠球菌ATCC33186      冻干粉                                         

QT-00053 空肠弯曲菌ATCC33291 冻干粉