1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！【人8异前列腺素α（8-ISO-PGα）elisa试剂盒现货】

ELISA应用：

ELISA检测试剂盒、ELISAKit、酶联免疫试剂盒、酶联免疫吸附测定试剂盒、酶联免疫分析试剂盒、酶免试剂盒等,比较常见的叫法是ELISA检测试剂盒、酶联免疫吸附测定试剂盒等ELISA试剂盒自从60-70年代问世以来,得到全世界科研工作者的认可及推崇,在欧美及中国获得很大的推广,尤其是国内生化领域的长足发展.Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法.由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检验的各领域中.ELISA检测试剂盒适用于体外定性检测人血清或血浆中的抗人类戊型肝炎（HEV）病毒IgM抗体ELISA检测

【人8异前列腺素α（8-ISO-PGα）elisa试剂盒现货】齐一生物销售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人8异前列腺素α（8-ISO-PGα）elisa试剂盒现货】ELISA实验原理

试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测.先将生物素标记的抗体同时温育.洗涤后,加入亲和素标记过的HRP.再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用.产生颜色.颜色的深浅和样品中的浓度呈比例关系.

免疫测定技术的基础在于抗原抗体之间的特异结合反应,所以任何优质的诊断试剂离不开优质的原料,如抗原抗体,酶等.以前用于免疫测定的抗原通常为各种纯化抗原,而抗体则为纯化抗原免疫动物后获得的多克隆抗体和使用杂交瘤技术得到的单克隆抗体,而抗原或抗体的标记物如酶标结合物则通过各种化学合成方法制备.近年来,随着分子生物学的发展,使用基因工程方法制备各种特殊的抗原或抗体及其酶结合物等免疫测定试剂几成为现实的新一代试剂,各种新型使用的测定方法也不断出现.

HBsAg试剂特点（检测模式：临床抗体夹心法）：包被抗体为山羊多抗.多抗具有高亲和性和对抗原各种表位的反应性.酶表抗体为与HBsAg有不同结合位点的鼠复合单位,可测得HBsAg变异样品,提高检测的特异性（99.98%）提高检测的灵敏度,应用ParlEhrich学说（PEI）HBsAg标准品,对ad标准品的灵敏度为0.05ng/ml对ay标准品灵敏度为0.025ng/ml

ELISA检测试剂盒应用定性夹心免疫检测技术,用合成的HEV多肽抗原包被微孔板板条,这些多肽是中国型HEV毒株核心氨基酸序列中抗原性很强的肽段,分别来自于该毒株的开放阅读框2和开放阅读框3.将样品或标准品加入孔中并孵育,如果其中存在HEVIgM抗体,这些抗体就会与HEV的多肽抗原结合,并固定在上面,洗板除去其它非特异性抗体和样品中的其它成份.然后加入羊抗人IgM-HRP（辣根过氧化物酶）酶结合物,经第二次孵育后,酶结合物就会与次孵育结合上的HEVIgM抗体相结合,洗板除去未结合的酶结合物,加入TMB底物溶液,在第三次孵育时会发生酶-底物反应,只有那些含有HEVIgM抗体和酶结合物所形成的复合物的孔才会发生颜色变化,加入硫酸溶液终止酶和底物间的反应,并在波长:450nm处测量O.D.值,按照本HEVIgM抗体elisa试剂盒的测试标准,O.D.值大于或等于Cut-Off值的样品被认为是初试阳性.

以上资料仅供参考了解，不供于论文写作

【人8异前列腺素α（8-ISO-PGα）elisa试剂盒现货】齐一生物专业为您提供高品质，高灵敏度.高质量的酶联免疫试剂盒，齐一生物长期经营ELISA试剂盒（免费代测）及抗体、分子学生物试剂盒、金标法检测试剂盒、比色法检测试剂盒、细胞菌株、培养基、细胞因子、生化试剂、耗材等生物试剂产品。齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

5000-1430   MST Vortexer II - FoamFeetAssembly, 110V        CNY            

5128-1220   Specimen Transport Medium (STM) CE      CNY            

5123-1220   Female Swab Specimen Collection KitTM       CNY            

5100-1400IVD    hc2 Sample Conversion Kit       CNY            

EA11424 EASYartus BKV RG_II     CNY            

5050-1075 V.1   CD Rom Manual, MST Vortexer 2       CNY            

EA10124 EASYartus EBV RG PCR Kit 2      CNY            

BR-02   DxS B-RAF Mutation Test Kit     CNY            

9240729 Tubing, 3/8"OD , Red, AP        CNY            

9240719 Cable Carrier, Capper, Elevator, AP     CNY            

9240701 O-Ring, Shaker, Lower Body, AP96/196        CNY             【人8异前列腺素α（8-ISO-PGα）elisa试剂盒现货】

9240696 Fitting, Barbed, 1/4" ID tube, AP       CNY            

9243651 Comprehen Valid., QIAgility """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   For planning purposes ａnd to ensure a smooth process, the Customer is expected to have all materials on-hand ａnd inventoried one (1) week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """ CNY            

218076  miScript SYBR Green PCR Kit (2000)      CNY            

271906  cattletype Milk Prep Kit (50)       CNY            

289980  intype IC-DNA (1ml)     CNY            

280437  virotype BTV RT-PCR Kit (480)       CNY            

932836  #QIAsymphony Ni-NTA Denat. Kit (384)        CNY            

800511  Verification Sequencing / DNA production        CNY            

9238243 ZLV BioRobot M96 User Manual        CNY            

9019988 ZLV Mainboard, QGY      CNY            

9020553 Foam angle, transport box, QIAC     CNY            

9020582 X-Motor, DML3000        CNY            

9020584 Y-Belt, DML3000 320 Teeth       CNY            

9020594 Enclosure, DML3000      CNY            

9021006 Motor, Pipettor Y, DCU      CNY            

9236333 Sample Loop     CNY             【人8异前列腺素α（8-ISO-PGα）elisa试剂盒现货】

9013382 Shaker adapter, microplate, LHS     CNY            

9013275 Motor, gripper, handler T/L, BR3000     CNY            

9013269 Fitting, PA tubing M6x1, 2.25mm, (10)       CNY            

9013210 Cabling kit, moving, BR8000     CNY            

QY-11612    anti-Clostridium perfringens type D兔抗D型产气荚膜梭菌抗体                    

QY-11622    Anti-C-Mer/MERTK (proto-oncogene tyrosine kinase)c-mer原癌基因酪氨酸激酶抗体                       

QY-11632    Anti-C-Met/HGFR（Hepatocyte growth factor receptor）肝细胞生长因子受体(原癌基因蛋白)抗体                       

QY-11642    Anti-C-Myc/MYC/MTLC致癌基因抗体                    

QY-11652    anti-c-Myc tagc-Myc tag标签抗体                    

QY-11662    Anti-CNP/CNPase ( 2＇,3＇-cyclic-nucleotide 3＇-phosphodiesterase)C型钠尿肽抗体                       

QY-11672    anti-CNTF（Ciliary Neurotrophic Factor）睫状神经营养因子抗体                       

QY-11682    Anti-Collagen Ⅱ(Anti-Collagen Type Ⅱ)抗Ⅱ型胶原抗体(Collagen Ⅱ Ⅱ型胶原)                    

QY-11692    Anti-Collagen Ⅲ(Anti-Collagen Type Ⅲ)抗Ⅲ型胶原抗体(Collagen Ⅲ Ⅲ型胶原)                    

QY-11702    Anti-Collagen Ⅳ(Anti-Collagen Type Ⅳ)抗IV型胶原抗体(Collagen Ⅳ Ⅳ 型胶原)                      

QY-11712    Anti-Collagen Ⅵ（Collagen Type Ⅵ)抗Ⅵ型胶原抗体                      

QY-11722    Anti-Collagen I(Anti-Collagen Type I)抗I型胶原抗体(I型胶原)                       

QY-11732    Anti-CollagenⅤ(Anti-Collagen Type Ⅴ)抗Ⅴ型胶原抗体(CollagenⅤ Ⅴ型胶原)                      

QY-11742    Anti-CollagenⅩ(Collagen TypeⅩ)抗Ⅹ型胶原抗体                      【人8异前列腺素α（8-ISO-PGα）elisa试剂盒现货】

QY-11752    Anti-COX-1 (Prostaglandin-endoperoxide synthase-1)前列腺素氧化环化酶1抗体                     

QY-11762    Anti-COX-2 (Prostaglandin-endoperoxide synthase-2)前列腺素氧化环化酶2抗体                     

QY-11772    Anti-C-PC(c-phycocyanin )C-藻蓝素/藻蓝蛋白抗体                     

QY-11782    Anti-C-Peptide（ C-PEP ）C-肽                      

QY-11792    Anti-CR (Calretinin)钙结合蛋白抗体                     

QY-11802    Anti-C-reactive protin CRPC-反应蛋白抗体                       

QY-11812    Anti-C-CRP（Mouse Anti-Human reactive protin CRP Antibody）鼠抗人C-反应蛋白单克隆抗体                      

QY-11822    Anti-CREB-1(cAMP responsive element binding protein-1)环腺苷酸应答元件结合蛋白抗体                     

QY-11832    Anti-CREB-1(cAMP responsive element binding protein-1)环腺苷酸应答元件结合蛋白抗体                     

QY-11842    Anti-P-CREB-1(p-cAMP responsive element binding protein-1)磷酸化环腺苷酸应答元件结合蛋白抗体                      

QY-11852    Anti-CRF (Corticotropin-Releasing Factor)促肾上腺皮质激素释放因子                      

QY-11862    Anti-CRF (Corticotropin-Releasing Factor)促肾上腺皮质激素释放因子                      

QY-11872    anti-CSP (circumsporozoite protein/Plasmodium yoelii)环子孢子蛋白(约氏疟原虫)抗体                      

QY-11882    Anti-CT （Calcitonin )降钙素抗体                       

QY-11892    Anti-CT DNA (culf thymus DNA)兔抗小牛胸腺DNA 抗体                      

QY-11902    Anti-CTGF (connective tissue growth factor)结缔组织生长因子抗体                     【人8异前列腺素α（8-ISO-PGα）elisa试剂盒现货】

QY-11912    Anti-CTLA-4/CD152（cytotoxic T-lymphocyte associated antigen-4）淋巴细胞相关抗原4/细胞毒性T细胞抗原-4抗体                       

QY-11922    anti-CTn1 (cardiac troponin 1)心肌肌钙蛋白抗体                     

QY-11932    Anti-CT-R（Calcitonin receptor）降钙素受体抗体