【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【人柠檬酸水解酶(ACL)试剂盒现货】样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人柠檬酸水解酶(ACL)试剂盒现货】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【人柠檬酸水解酶(ACL)试剂盒现货】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【人柠檬酸水解酶(ACL)试剂盒现货】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

51183      QIAamp DNA Blood Midi Kit (20) For 20 DNA midipreps: 20 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml)       CNY                           

9015518  Cable bare, Y         CNY                           

9001885  QIAcube (230V) System       QIAcube (230V) System combines the QIAcube system ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with a training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.    CNY                           

9001873  EZ1 Advanced, PrioPLUS      "EZ1 Advanced Instrument

Installation of EZ1 instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"   CNY                           

9001869  Rotor-Gene Q 6plex (PC) Priority Rotor-Gene Q 6plex (PC) Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service ａnd response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents. CNY                           

9018449  Piston, locate (1), QX           CNY                           

9015450  Upgrade kit, support sheet, DISU v2.1         CNY                           

9015448  Pump, dispenser, BR8000, v2.0           CNY                           

9015447  Modules, DISU, BR8 RD v2.0             CNY                           

9015409  Belt, Y-timing, v2, M96         CNY                           

291053    mericon GMO Screen CTP2-CP4EPSPS (24)            CNY                            【人柠檬酸水解酶(ACL)试剂盒现货】

9015387  Maintenance Kit, BR MDx            CNY                           

9001850  QIAsymphony RGQ System         CNY                           

9012291  Reagent holder tray        CNY                           

133077    NeXtalStock PEG 2,000 (200)             CNY                           

133076    NeXtalStock PEG 10,000 (200)           CNY                           

9015380  Unit, heat/cool, left, M96              CNY                           

9015373  Magnet, M96         CNY                           

9015371  Plungers, M96 (8)         CNY                           

133154    NeXtalStock BIS-TRIS Propane pH6.5 (100)             CNY                           

133153    NeXtalStock BIS-TRIS pH 8.5 (100)           CNY                           

204545    QuantiTect Multiplex PCR Kit (1000)    "1x 25 ml Master Mix

1x 20 ml RNase free water"   CNY                           

133062    NeXtalStock Magnesium nitrate (200)         CNY                           

133061    NeXtalStock Magnesium formate (200)              CNY                           

870011    therascreen KRAS RGQ PCR Kit CE (24)           CNY                           

290055    mericon VTEC stx1/2 Kit (96)             CNY                           

204341    QuantiTect Probe PCR Kit (40)     "-Trial kit size: 40 x 50 µl rxns at an economical price

- 1x 1ml 2x Master Mix, 1x 2 ml RNase free water

For quantitative, real-time PCR ａnd two-step RT-PCR using sequence-specific probes."   CNY                            【人柠檬酸水解酶(ACL)试剂盒现货】

9018392  Key, Data Review, grey, QIAxcel FA           CNY                           

9012271  Power Supply, 24V / 8Amp, (9194)             CNY                           

9001647  Rotor-Gene Q 5plex (w/o PC) System  Rotor-Gene Q 5plex (w/o PC) System includes the Rotor-Gene instrument ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It covers the handling of the Rotorgene-Q systems hardware ａnd trains the user on operation of the Rotorgene software. All travel ａnd labor cost included for Installation ａnd service documented on a GMP/GLP-compliant Field Service document.     CNY                           

9015332  Sensor, pressure, M48          CNY                           

 QY-03060       抗碱性磷酸酶抗体       Anti-AP/ALP(Alkaline phosphatase)                                

 QY-03061       α－甲基酰基辅酶A消旋酶抗体      Anti-AMACR/P504S(alpha-methylacyl-CoA racemase)                                 

 QY-03062       神经元突触前膜蛋白抗体    Anti-Amph(Amphiphysin)                                

 QY-03063       腺苷单磷酸活化蛋白激酶α1抗体     Anti-AMPK alpha-1 (AMP-activated Protein Kinase-α1)                              

 QY-03064       腺苷单磷酸活化蛋白激酶β1抗体     Anti-AMPKβ1 (AMP-activated Protein Kinase beta-1)                                 

 QY-03065       糖尿病相关肽/胰岛淀粉样肽抗体      Anti-Amylin                              

 QY-03066       β淀粉样肽1-42 (C端抗体)       Anti-β-Amyloid 1-42（Aβ1-42）(CT)                                

 QY-03067       β淀粉样肽（1-16）抗体    Anti-β-Amyloid（1-16）                               

 QY-03068       β淀粉样肽（31-35）抗体  Anti-β-Amyloid（31-35）                              

 QY-03069      β淀粉样肽（1-28）抗体   Anti-β-Amyloid（1-28）                               

 QY-03070       小鼠抗β淀粉样肽（1-28）抗体        Anti-β-Amyloid（1-28）                               

 QY-03071       β淀粉样肽（25-35）抗体  Anti-β-Amyloid（25-35）                              

 QY-03072       β淀粉样肽（1-40）抗体    Anti-β-Amyloid（1-40)                                  

 QY-03073       β淀粉样肽β-Amyloid 1-40/Aβ1-40 C端抗体        Anti-β-Amyloid 1-40/Aβ1-40（CT）                                   【人柠檬酸水解酶(ACL)试剂盒现货】

 QY-03074       β淀粉样肽 1-40 C端抗体  Anti-β-Amyloid 1-40（CT）mouse                               

 QY-03075       β淀粉样肽（1-42）抗体    Anti-β-Amyloid（1-42）                               

 QY-03076       血管生成素-1抗体       Anti-ANG-1/(Angiopoietin 1; angiopoietin-like 4)                            

 QY-03077       血管生成素-2抗体       Anti-ANG-2 (Angiopoietin 2)hu, mo, rat, pig, dog, chi                                  

 QY-03078       锚定蛋白G抗体   Anti-Ankyrin G/ANK-3/ankyrin 3                            

 QY-03079       心钠肽（心钠素）抗体       Anti-ANP(atrial natriuretic peptide)                                 

 QY-03080       血管紧张素Ⅱ-2型受体抗体       Anti-AT2R（Angiotensin Ⅱ Type 2 Receptor）                                  

 QY-03081       血管紧张素II抗体       Anti-AT-Ⅱ (Angiotensin Ⅱ)                                  

 QY-03082       血管位蛋白样2/血管生成素样蛋白-2抗体       Anti-ANGPTL2/ANG-2 (Angiopoietin-like Protein 2)                             

 QY-03083       血管位蛋白样4/血管生成素样蛋白-4抗体       Anti-ANGPTL4/ANG-4 (Angiopoietin-like Protein 4)                             

 QY-03084       膜粘连蛋白A1抗体     Anti-ANXA1/P35(Annexin A1)                                

 QY-03085       膜粘连蛋白 I抗体       Anti-Annexin I                                  

 QY-03086       膜粘连蛋白 Ⅱ抗体     Anti-Annexin Ⅱ                               

 QY-03087       膜粘连蛋白 Ⅲ抗体     Anti-Annexin Ⅲ                               

 QY-03088       膜粘连蛋白 Ⅳ抗体     Anti-Annexin Ⅳ                               

 QY-03089       膜粘连蛋白 V抗体      Anti-Annexin V                                  【人柠檬酸水解酶(ACL)试剂盒现货】

 QY-03090       膜粘连蛋白 VI抗体     Anti-Annexin VI                               

 QY-03091       重组膜粘连蛋白-5(人)抗体  Anti-Annexin V (Recomb Humen Annexin V)                                 

 QY-03092       炭疽菌抗体    Anti-Anthrax