Validated, Complete Assay for the Sensitive Detection of a Diverse Set of PEGs in Biological Samples

•Validated for a wide range of MW linear ａnd branched PEGs, both in free form ａnd when conjugated to proteins

•Highly sensitive, measure as low as <1ng/mL of PEGylated proteins

•Quantify PEGylated target molecules in complex matrices to monitor drug levels or its accumulation in tissue

•High-throughput format, analyze up to 35 samples in duplicate results in just 2 hours

The PEGylated Protein ELISA kit is a competitive assay specific to the backbone of poly(ethylene glycol) (PEG) ａnd has been validated for use with a wide variety of PEG molecules including linear ａnd branched forms as well as free ａnd conjugated forms. As expected, the assay is more sensitive to higher molecular weight PEG molecules due to the repeating characteristics of the backbone. The assay uses a monoclonal antibody to PEG pre-coated on a microtiter plate. The antibody binds in a competitive manner the PEG or PEGylated protein in the sample or the PEG covalently linked to biotin. After incubation at room temperature the excess reagents are washed away ａnd a streptavidin HRP conjugate is added to bind to the biotinylated PEG bound to the antibody. After a second wash to remove excess HRP substrate is added. An HRP-catalyzed reaction gener-ates a blue color in the solution. The reaction is sped with s solution ａnd the resulting yellow color is read at 450nm. The amount of signal is inversely proportional to the concentration of PEG.

Product Details

Alternative Name: Polyethylene glycol

Sensitivity:  <1 ng/ml (range 1.75-225 ng/ml)

Assay Time:  2 hours

Applications:  ELISA, Colorimetric detection

Application Notes: For the measurement of PEGylation ａnd quantitative determination of PEGylated molecules in plasma, serum, tissue ａnd other biological samples.

Wavelength:  450 nm

Species reactivity: Species independent

Shipping: Blue Ice Not Frozen

Long Term Storage:  +4°C

Contents: Microtiter Plate, PEG-BSA Assay Control, Biotinylated PEG, Assay Buffer 39, Conjugate, Wash Buffer 3, TMB Substrate, S Solution 2, Plate Sealer

艾美捷 聚乙二醇化蛋白质 ELISA试剂盒是种针对聚乙二醇 (PEG) 骨架的竞争性检测试剂盒，已被验证可用于多种 PEG 分子，包括直链和支链形式以及游离和共轭形式。正如预期的那样，由于骨架的重复特征，该测定对更高分子量的 PEG 分子更敏感。该测定使用预涂在微量滴定板上的针对 PEG 的单克隆抗体。抗体以竞争方式结合样品中的 PEG 或 PEG 化蛋白或与生物素共价连接的 PEG。在室温下孵育后，洗去多余的试剂并加入链霉亲和素 HRP 偶联物以结合与抗体结合的生物素化 PEG。在第二次洗涤以去除多余的 HRP 底物后，添加。HRP 催化的反应在溶液中产生蓝色。用终止溶液终止反应并在 450nm 处读取所得黄色。信号量与 PEG 浓度成反比。

艾美捷 PEG化蛋白ELISA试剂盒特点：

1.经验证适用于各种 MW 线性和支链 PEG，无论是游离形式还是与蛋白质结合时

2.高度灵敏，可测量低至 <1ng/mL 的聚乙二醇化蛋白质

3.量化复杂基质中的 PEG 化靶分子，以监测药物水平或其在组织中的积累

4.高通量格式，只需 2 小时即可分析多达 35 个样品，重复结果

艾美捷PEG化蛋白ELISA试剂盒化学性质：

备用名称：聚乙二醇

灵敏度：<1 ng/ml（范围 1.75-225 ng/ml）

检测时间：2小时

应用：ELISA，比色检测

应用说明：用于测量血浆、血清、组织和其他生物样品中的聚乙二醇化分子和定量测定聚乙二醇化分子。

波长：450 纳米

物种反应性：物种独立

船运：蓝冰未冷冻

长期储存：+4°C

内容：微量滴定板、PEG-BSA 检测对照、生物素化 PEG、检测缓冲液 39、偶联物、洗涤缓冲液 3、TMB 底物、终止液 2、封板剂

RUO - 仅供研究使用

来源：https://www.amyjet.com/products/ADI-900-213-0001.shtml