INTRODUCTION

Beta 2 microglobulin is an 11 kDa protein associated with the outer membrane of many cells including lymphocytes. It is the small subunit of the MHC class I molecule. Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. Beta 2 microglobulin associates with class I-like molecules such as CD1 ａnd Qa as well as with the alpha chain of MHC class I molecules. Very limited amounts of MHC class I molecules can be found on the surface in the absence of Beta 2 microglobulin. CD8 T cells cannot develop in the absence of MHC class I.

Beta 2-microglobulin is present in small amounts in serum, csf, ａnd urine of normal people, ａnd to a much greater degree in the urine ａnd plasma of patients with tubular proteinaemia, renal failure, or kidney transplants. Human Beta 2 microglobulin levels can rise either because its rate of synthesis has increased (e.g. in AIDS, malignant monoclonal plasma cell dyscrasia, solid tumors ａnd autoimmune disease) or because of impaired renal filtration (e.g. due to renal insufficiency, graft rejection or nephrotoxicity induced by post-transplantation immunosuppressive therapy). Beta-2 microglobulin levels might also be elevated in multiple myeloma ａnd lymphoma cases. Dialysis-related amyloidosis develops after a long-term hemodialysis, it can aggregate into amyloid fibers that deposit in joint spaces.

DESCRIPTION

Human Beta-2 Microglobulin produced in Human urine from patients with tubular proteinuria having a molecular mass of 12KDa.

SOURCE

Human urine from patients with tubular proteinuria.

PHYSICAL APPEARANCE

Sterile Filtered White lyophilized (freeze-dried) powder.

FORMULATION

Lyophilized from 0.02M NH4HCO3.

SOLUBILITY

It is recommended to reconstitute the lyophilized HuB2M in phosphate buffer pH > 7.0 containing 0.15M NaCl.

STABILITY

Human B2M although stable at room temperature for 3 weeks, should be stored between 2-8°C.

PURITY

Greater than 98.0%.

HUMAN VIRUS TEST

Starting material tested ａnd found negative for HIV I & II antibodies, Hepatitis B surface antigen, Hepatitis C antibodies ａnd Syphilis.

SAFETY DATA SHEET

SDS

USAGE

Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

艾美捷人β2微球蛋白中文说明书：

人β2微球蛋白是一种11kDa蛋白，与包括淋巴细胞在内的许多细胞的外膜相关。它是MHC I类分子的小亚单位。与β2-微球蛋白结合通常是I类重链从内质网运输到细胞表面所必需的人β2微球蛋白与I类分子如CD1和Qa以及MHC I类分子的α链相关。在没有人β2微球蛋白。CD8 T细胞在没有MHC I类的情况下不能发育。

β2-微球蛋白在正常人的血清、脑脊液和尿液中少量存在，在肾小管蛋白血症、肾衰竭或肾移植患者的尿液和血浆中的含量更高。人β2微球蛋白水平可能会升高，因为其合成速度加快（例如在艾滋病、恶性单克隆浆细胞疾病、实体瘤和自身免疫性疾病中），或者因为肾过滤功能受损（例如由于肾功能不全、移植排斥或移植后免疫抑制治疗引起的肾毒性）。在多发性骨髓瘤和淋巴瘤患者中，β-2微球蛋白水平也可能升高。透析相关淀粉样变在长期血液透析后发生，它可以聚集成淀粉样纤维沉积在关节间隙。

艾美捷人β2微球蛋白化学性质：

编号：PRO-553

同义词：Beta-2-微球蛋白，B2M。

资源：管状蛋白尿患者的人尿。

形态：无菌过滤的白色冻干（冷冻干燥）粉末。

公式：从 0.02M NH4HCO3 冻干。

溶解度：建议在含有 0.15M NaCl 的磷酸盐缓冲液 pH > 7.0 中重构冻干的 HuB2M。

稳定：人 B2M 虽然在室温下可稳定保存 3 周，但应在 2-8°C 之间储存。

纯度：大于 98.0%。

人类病毒测试：对起始材料进行测试，发现 HIV I 和 II 抗体、乙型肝炎表面抗原、丙型肝炎抗体和梅毒呈阴性。

仅供实验室研究使用。不得用作药物、农业或农药产品、食品添加剂或家用化学品。

来源：https://www.amyjet.com/products/PRO-553.shtml