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中英文说明丨艾美捷血管紧张素ⅡELISA试剂盒方案

点击次数:0发布时间:2023/1/17 16:55:22

中英文说明丨艾美捷血管紧张素ⅡELISA试剂盒方案

更新日期:2023/1/17 16:55:22

所 在 地:中国大陆

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简单介绍:血管紧张素Ⅱ ELISA试剂盒:高灵敏度ELISA,检测量低至4.6pg/ml

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详细内容

 Angiotensins are small peptides derived from angiotensinogen. Several of the known angiotensins are established endocrine effectors in the regulation of blood pressure, but they are also known to have other functions locally, in several organs and tissues (paracrine) and at the cellular level (autocrine / intracrine). Angiotensin I (DRVYIHPFHL) has no known effector function but it is an immediate precursor of Angiotensin II (DRVYIHPF). Angiotensin II binds AT1 receptors, which promote vasoconstriction, sodium retention, release of aldosterone, release of Arg-vasopressin, cell proliferation, inflammation, fibrosis, anxiety, and cardiac hypertrophy. Angiotensin A (ARVYIHPF), and Angiotensin III (RVYIHPF), also bind AT1 receptors. Angiotensin (1-7) (DRVYIHP) binds a different receptor called MAS-1 which has opposite effects (vasodilation, natriuresis, antiproliferation, NO release, PGE release, and apoptosis). Angiotensin IV (VYIHPF) binds yet another receptor called AT4 (IRAP), which promotes increase of blood flow, angiogenesis, and natriuresis, and which has also been implicated in memory formation and in the pathogenesis of Alzheimers disease. The peptide LVV-hemorphin-7 (LVVYPWTQRF), which is not an angiotensin, also binds the AT4 receptor. There are other angiotensin peptides that have been identified, including Ang (1-9) (DRVYIHPFH), Ang (1-12) (DRVYIHPFHLVI), Ang V(3-7) (VYIHP), as well as several other shorter peptides that have undetermined functions.

Sensitivity: 4.6 pg/ml (range 3.9-10,000 pg/ml)

Assay Time: 3.5 hours

Applications: ELISA, Colorimetric detection

Application Notes: For the quantitative determination of species independent Angiotensin II in plasma and serum samples.

Wavelength: 450 nm

Species reactivity: Species independent

Crossreactivity: Angiotensin II (100%), Angiotensin A (100%), Angiotensin III (100%), Angiotensin IV (100%), Angiotensin I (0.319%), Angiotensin (1-9) (0.103%), LVV-hemorphin 7 (0.067%), Angiotensin (1-7) (0.053%), Bradykinin (0.022%)

Quantity: 1 x 96-well plate

Use/Stability: Store all components at +4ºC, except Standard at -20ºC.

Shipping: Shipped on Blue Ice

Contents: Microtiter Plate, Conjugate, Antibody, Assay Buffer 16, Wash Buffer Concentrate, Standard, TMB Substrate, S Solution 2, Streptavidin-HRP

 

ADI-900-204血管紧张素ⅡELISA试剂盒:高灵敏度ELISA,检测量低至4.6pg/ml

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艾美捷ADI-900-204 血管紧张素ⅡELISA试剂盒化学性质:

灵敏度:4.6 pg/ml(范围3.9-10000 pg/ml

化验时间:3.5小时

应用:ELISA,比色检测

应用说明:用于定量测定血浆和血清样品中的种类无关的血管紧张素II

波长:450 nm

物种反应性:物种无关

交叉反应性:血管紧张素II100%),血管紧张素A100%)、血管紧张素III100%)和血管紧张素IV100%

数量:1 x 96孔板

使用/稳定性:将所有组件储存在+4ºC,标准组件除外,储存在-20ºC

运输:蓝冰运输

内容:微量滴定板、缀合物、抗体、测定缓冲液16、洗涤缓冲液浓缩液、标准品、TMB底物、停止溶液2、链霉亲和素HRP

 

血管紧张素ⅡELISA试剂盒文献参考:

Hypertension enhances advanced atherosclerosis and induces cardiac death in Watanabe heritable hyperlipidemic rabbits: B. Ning, et al.; Am. J. Pathol. 188, 2936 (2018);

Post-translational modifications of eNOS augment nitric oxide availability and facilitates hypoxia adaptation in Ladakhi women: Pooja, et al.; Nitric Oxide 78, 103 (2018), Application(s): ELISA using human plasma;

Prorenin independently causes hypertension and renal and cardiac fibrosis in cyp1a1-prorenin transgenic rats: G. Zhou, et al.; Clin. Sci. (Lond.) 132, 1345 (2018);

12-Lipoxygenase Inhibition on Microalbuminuria in Type-1 and Type-2 Diabetes Is Associated with Changes of Glomerular Angiotensin II Type 1 Receptor Related to Insulin Resistance: H.Z. Xu, et al.; Int. J. Mol. Sci. 17, E684 (2016), Application(s): Measurement of Angiotensin levels in rat;

 

来源:https://www.amyjet.com/products/ADI-900-204.shtml

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