SiR Action cell imaging

The imaging of SiR action is best done using standard Cy5 settings. After labeling, live cells can be immediately imaged without the need for washing steps. Optionally, a simple washing step includes replacing the labeled solution with fresh culture medium without probes, which typically improves the signal-to-noise ratio. If time elapses for imaging, it is recommended to keep the probe concentration equal to or lower than 100 nM throughout the experiment to obtain a constant signal ａnd avoid probe interference with actin dynamics (reducing cell proliferation). If cleaning cells

Before imaging, the staining will last for several hours.

SiR-actin具有荧光性、细胞通透性和对F-actin的高度特异性。Sir-actin染色内源性的F-actin不需要基因操作或过表达。它在远红光中的发射大限度地降低了光毒性和样品的自身荧光。SiR-actin兼容GFP和/或m-cherry荧光蛋白质。它可以用标准的Cy5滤镜进行成像。SiR-actin可用于活细胞和组织的宽视场、共聚焦、SIM或STED成像。探针数量允许50 - 200染色实验。*（*基于以下条件:0.5 - 1ml染色液/ 0.5 - 1um探针浓度的染色实验。染色实验的数量可以通过减少体积或探针浓度来进一步增加）。

艾美捷 SiR-Actin试剂盒#CY-SC001物理特性：

abs 652 nm

Em 674 nm

ε652 nm 1.0·105 mol-1·cm-1

MW 1241.6 g/mol

MF C71H88N8O10Si

探针浓度（nM） 建议标记时间（h）*

> 1000      0.5 - 1

500           3 - 4

200           4 - 6

< 100        6 - 12

SiR-Actin试剂盒文献参考：

1. Fluorogenic probes for live-cell imaging of the cytoskeleton, G. Lukinavičius et al., Nature Methods, 11, 731–733 (2014)

来源：https://www.amyjet.com/products/CY-SC001.shtml