1.购买我公司任意ELISA试剂盒指标享受免费代测，并提供技术指导服务！

二、实验材料与试剂配制：

1. 仪器与材料：酶标仪（使用前预热30分钟），微量加液器、吸头、蒸馏水或去离子水，滤纸。

2. 缓冲液使用：加5ul  的缓冲液于50ul  的样品中，如果样品量不够或者不确定，缓冲液和样品的混合比例不要小于1:10即可混匀，静置1小时备用（如果标本是血清或者血浆，此步骤忽略）。

3. 洗液的配制：按1:100的比例配制洗液备用。

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人过氧化物酶体增殖激活物受体γ辅激活因子1α(PPARGC1)ELISA试剂盒厂家】三. 仔细阅读说明书

1.检查试剂盒标签上的有效期。如果超过有效期，请勿使用。

2. 按说明书确定所有试剂齐全（数量、体积）

3. 标本制备要规范，每份标本体积要按2-3个复孔以上的量制备（贮存），尽量分装做备份。

4.短期内无法实验者，注意低温保存

5.准备好所有实验额外所需物品，（比如移液器，试管，清洗器，酶标仪）

6. 按说明书将所用试剂平衡至室温，根据检测标本数量确定所需试剂的量

7.检查试剂盒内每种试剂的贮存条件，保证所有试剂均按照试剂盒内说明书推荐的条件存储。 【人过氧化物酶体增殖激活物受体γ辅激活因子1α(PPARGC1)ELISA试剂盒厂家】

8.检查不稳定或者变质的试剂溶液（比如沉淀或变色）。有些试剂，比如标准品稀释液或者检测稀释液，可能在设计时就含有沉淀物。所有试剂在滴入酶标板之前，必需充分混匀。而由于沉淀物的特性，在加入酶标板之前，必需不停搅拌。

9.保证充分的孵育时间和温度。

10.切勿使用不同生产批号的试剂取代现有试剂或混合现有试剂。

11.在混合或溶解蛋白溶液时，避免泡沫产生。

12.在实验开始之前，安排好实验流程。在实验开始之前，清洁工作台。

13.若有问题，应与我公司或代理商的技术支持联系

【人过氧化物酶体增殖激活物受体γ辅激活因子1α(PPARGC1)ELISA试剂盒厂家】四、客户须知

齐一生物销售：021－6034 8496；181214 53965；173021 04490

1.在收集标本前都必须有一个完整的计划，必须清楚要检测的成份是否足够稳定。我们提倡新鲜标本尽早检测，对收集后当天就进行检测的标本，及时储存在4℃备用，如有特殊原因需要周期收集标本，请造模取材后，将标本及时分装后放在-20℃或-70℃条件下保存。因冰室与室温存在一定温差，蛋白极易降解，直接影响实验质量，所以避免反复冻融。部分标本也可加入适当防腐剂，激素类标本需添加抑肽酶。 

2.试剂盒代测标本的客户取材前须向我司销售人员索要说明书，具体操作注意事项请与我司技术人员沟通。

3.液体类标本：标本必须为液体，不含沉淀。包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等。

4.购买前请提前告诉我们：您样本的种属、样本数量、待测指标及其它特殊要求。

【人过氧化物酶体增殖激活物受体γ辅激活因子1α(PPARGC1)ELISA试剂盒厂家】五、代测数据详细内容

1、ELISA标准曲线；

2、实验具体详细步骤；

3、完整的实验报告（试剂耗材，实验方法，实验结果及结果说明）；

4、实验进行阶段，我公司客服人员会及时向您汇报实验进程。

9237106  Protocol Dev./Opt., on-site (2-days)         The QIAGEN BioRobot Systems Protocol Development/Optimization On-Site Visit includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Instrumentation Service Specialist to write and/or opimize protocols for the BioRobot System. Parts, consumable chemistries, ａnd accessories not included. Custom protocols are the sole responsibility of the customer ａnd neither warrantied nor guaranteed.          CNY                             

9237097  Training, BR QIAGEN Advanced       A minimum of 4 people are required to attend. The QIAGEN BioRobot Systems Advanced Training includes three days of  training at our training facility to introduce users to advanced software topics for the  QIAGEN BioRobot Systems.  Designed for users with a basic understanding of the use ａnd programming of QIAGEN BioRobot systems who want to write complex protocols for their instrument, the advanced training starts with a brief review of basic concepts such as teaching slots ａnd racks before exploring the higher-level programming features of the QIAsoft Operating System.  Topics include the structure ａnd use of functions, using variable for programming flexibility, IF/ELSE/END boolean logic commands, ａnd parameterized pipetting using tables.  Users will write, test ａnd run protocols they have developed to demonstrate ａnd reinforce training success.       CNY                             

9236422  Power supply, replacement kit, TWI                  CNY                             

9232968  Print YZ96-Adapter-Board Plato-7            CNY                             

9236363  Reservoir XYP            CNY                             

9236353  Tubing Sample Needle to 3-Way Valve              CNY                             

9236329  2-Way Valve (Sheath or vent)           CNY                              【人过氧化物酶体增殖激活物受体γ辅激活因子1α(PPARGC1)ELISA试剂盒厂家】

9236160  Potentionmeter, rotary, kit, RapidPlate           CNY                             

9236154  Online Fill Pump RP           CNY                             

9236126  QIAGEN Multiwell-Plate Holder , silver   Holder for accommodating multiwell plates on the Microplate station of the BioRobot MDx worktable         CNY                             

9236120  Runner, Drawer, BR8 RD                   CNY                             

9235790  Ramp, Locking, Drawer, BR8 RD              CNY                             

9230745  Z-Lead screw for Plato 7           CNY                             

9235690  Cable X-Main, RCS1           CNY                             

9235572  Shaker Tool, RCS1              CNY                             

9230314  Cable, RS232, syringe pump, 225mm                CNY                             

9230283  Cable, Interface, X-main to Washer                   CNY                             

5000-1302        Capacitor 2UF, SKR1                  CNY                             

5000-1284        DML 2000 - Power Cord, 220V D Type (GB)              CNY                             

5000-1254        Power Supply PCB, DML2                  CNY                             

5000-1250        CPU DML 2000 ASSRY Kit, DML2              CNY                             

6000-3401        RCS Upgrade Package               CNY                             

6000-3116        Plate Washer I - 105 Micron Filters          CNY                              【人过氧化物酶体增殖激活物受体γ辅激活因子1α(PPARGC1)ELISA试剂盒厂家】

6000-00183      Plate Washer - Manifold           CNY                             

6000-00177      Plate Washer - Syringe Assembly              CNY                             

5000-1501        Holder Fuse, HTR1            CNY                             

5135-1220        hc2 CT-ID DNA Test           CNY                             

BR-01       DxS B-RAF Mutation Test Kit            CNY                             

9240716  Lead Screw Assy, Capper U\D, AP196/98          CNY                             

9240712  Muffler, Elevator Brake, AP196/98            CNY                             

5000-1416        Switch LRGSK61RCBE250, VTX2                CNY                             

6000-5023        LumiCheck Plate User Package (CD Manual)            CNY                             

6000-3102        Rapid Capture System & Manuals            CNY                             

MCM        小鼠心肌细胞         Mouse Cardiac Myocytes         1 x 10^6 cells/vial                                

MCF 小鼠心脏成纤维细胞     Mouse Cardiac Fibroblasts       5 x 10^5 cells/vial                              

MMSC-bm        小鼠骨髓间充质干细胞         Mouse Mesenchymal Stem Cells-bone marrow       5 x 10^5 cells/vial                                

MEF-mt    小鼠胚胎成纤维细胞     Mouse Embryonic Fibroblasts 5 x 10^5 cells/vial                                 【人过氧化物酶体增殖激活物受体γ辅激活因子1α(PPARGC1)ELISA试剂盒厂家】

RPC  大鼠垂体细胞         Rat Pituitary Cells    5 x 10^5 cells/vial                                

RMC          大鼠脑膜细胞         Rat Meningeal Cells          5 x 10^5 cells/vial                                

RN-r 大鼠脑脊神经元     Rat Neurons-raphe  1 x 10^6 cells/vial                                

RN-c          大鼠皮质神经元     Rat Neurons-cortical        1 x 10^6 cells/vial                                

RGC 大鼠颗粒细胞         Rat Granule Cells     1 x 10^6 cells/vial                                

RN-h         大鼠海马趾神经元         Rat Neurons-hippocampal       1 x 10^6 cells/vial                                

RN-sn        大鼠黑质神经元     Rat Neurons-substantia nigra 1 x 10^6 cells/vial                                

RN-s 大鼠纹状体神经元         Rat Neurons-striatal         1 x 10^6 cells/vial                                

RN-vsc      大鼠腹脊髓神经元         Rat Neurons-ventral spinal cord      1 x 10^6 cells/vial                                

RN-dsc      大鼠背脊髓神经元         Rat Neurons-dorsal spinal cord        1 x 10^6 cells/vial                                

RN-sc        大鼠背脊髓神经元         Rat Neurons-dorsal spinal cord        1 x 10^6 cells/vial                              

ROPC        大鼠少突胶质前体细胞         Rat Oligodendrocyte Precursor Cells        1 x 10^6 cells/vial                                

ROPC-f      大鼠少突胶质前体细胞-新鲜        Rat Oligodendrocyte Precursor Cells-fresh      1 x 10^6 cells/tube                                     

RSC  大鼠雪旺细胞         Rat Schwann Cells    5 x 10^5 cells/vial                                

RPNF         大鼠神经束膜细胞         Rat Perineural Fibroblasts        5 x 10^5 cells/vial                                

RA    大鼠星形胶质细胞         Rat Astrocytes 1 x 10^6 cells/vial                                 【人过氧化物酶体增殖激活物受体γ辅激活因子1α(PPARGC1)ELISA试剂盒厂家】

RA-c 大鼠小脑星形胶质细胞         Rat Astrocytes-cerebellar        1 x 10^6 cells/vial                                

RA-h          大鼠海马趾星形胶质细胞     Rat Astrocytes-hippocampal   1 x 10^6 cells/vial                                

RM   大鼠小胶质细胞     Rat Microglia   5 x 10^5 cells/vial                                

RMa-bm  大鼠巨噬细胞         Rat Macrophage      1 x 10^6 cells/vial                              

REK-n        大鼠表皮角质细胞-新生        Rat Epidermal Keratinocytes-neonate    5 x 10^5 cells/vial                                

RDF-n       大鼠皮肤成纤维细胞 - 新生        Rat Dermal Fibroblasts - neonate   5 x 10^5 cells/vial                                

RDF-a       大鼠皮肤成纤维细胞-成年   Rat Dermal Fibroblasts-adult  5 x 10^5 cells/vial                                

RLF   大鼠淋巴成纤维细胞     Rat Lymphatic Fibroblasts        5 x 10^5 cells/vial                                

RPAEpiC   大鼠肺泡上皮细胞         Rat Pulmonary Alveolar Epithelial Cells   1 x 10^6 cells/vial                                

RPF  大鼠肺成纤维细胞         Rat Pulmonary Fibroblasts       5 x 10^5 cells/vial                                

RRTEpiC   大鼠肾小管上皮细胞     Rat Renal Tubular Epithelial Cells    5 x 10^5 cells/vial