标题Ampligase® DNA Ligase W/Buffer epicentre A32250

• 高热稳定性，保证可以使用更严谨的杂交温度。
• 高特异性和严谨性能够敏感检测SNPs.11

活力单位定义: 1个活力单位的Ampligase DNA Ligase指在1X Ampligase Reaction缓冲液中，45°C条件下1分钟能够催化1 µg的lambda DNA中的50% cos位点发生连接。 
Note:一个单位的Ampligase DNA Ligase等价于至少15个别处定义的 "cohesive end units"或 "nick ligation units"。4 
储存缓冲液: 50%甘油含有50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT和0.1% Triton® X-100。 
Ampligase 10X反应缓冲液: 200 mM Tris-HCl (pH 8.3), 250 mM KCl, 100 mM MgCl2, 5 mM NAD和0.1% Triton® X-100。 
质量控制: Ampligase Thermostable DNA Ligase的活性通过在45°C条件下连接Sma I消化后产生的平端噬菌体DNA进行检测；电泳检测连接只发生在cos位点。Ampligase DNA Ligase不含DNA外切、内切核酸酶和RNA酶活性。 
References

图例说明

Figure 2. Ligation Amplification using Ampligase? DNA Ligase. Oligonucleotides A, B, C, ａnd D from Figure 1 (25 pmoles each) were incubated as in Figure 1 with 5 units of Ampligase DNA Ligase in 25 ?l of 1X Ampligase Reaction Buffer, with (+) or without (-) 0.25 pmole DNA template. The reactions were cycled 40 times for 1 minute at 94°C ａnd 2 minutes at 65°C, then separated by electrophoresis ａnd visualized with ethidium bromide. In the presence of template DNA, the target sequence is amplified by formation of the ligation products A+B ａnd C+D.

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