lucigen 总代理 epicentre 2´-dCTP D5910C

dATP，dCTP，dGTP和dTTP的无菌中性pH溶液可以作为单个核苷酸或所有四种核苷酸的混合物获得，用于DNA合成。仅含有一种dNTP的溶液可以100mM浓度获得。含有所有四种dNTP的预混溶液也可以两种浓度，2.5mM和25mM的每种dNTP获得。在与大多数DNA聚合酶的典型反应中使用的浓度下，每种溶液足以进行至少1,000次50μl反应。无菌的，pH中和的20mM dUTP溶液也可用于合成含尿嘧啶的DNA。

储存缓冲液： dNTP溶液用无菌去离子水制备并调节至pH 7.0。

质量控制：所有EPICENTER的dNTP解决方案均符合分光光度标准，并使用我们的DNA聚合酶进行功能测试。

Sterile, neutral pH solutions of dATP, dCTP, dGTP, ａnd dTTP are available either as individual nucleotides or a mixture of all four nucleotides for use in DNA synthesis. Solutions containing only one dNTP are available in 100 mM concentrations. Premixed solutions containing all four dNTPs are also available at two concentrations, 2.5 mM ａnd 25 mM of each dNTP. Each solution is sufficient for at least 1,000 50-µl reactions at concentrations used in typical reactions with most DNA polymerases. A sterile, pH neutralized 20 mM solution of dUTP is also available for synthesis of uracil-containing DNA.

Storage Buffer: dNTP solutions are made with sterile deionized water ａnd adjusted to pH 7.0.

Quality Control: All of EPICENTRE＇s dNTP solutions meet spectrophotometric criteria ａnd are function-tested using our DNA polymerases.

dATP (2´-Deoxyadenosine-5´-Triphosphate)
Formula (free acid): C₁₀H₁₆N₅O₁₂P₃

Molecular weight: 491
A₂₅₀/A₂₆₀ = 0.80
A₂₈₀/A₂₆₀ = 0.15

dCTP (2´-Deoxycytidine-5´-Triphosphate)
Formula (free acid): C₉H₁₆N₃O₁₃P₃

Molecular weight: 467
A₂₅₀/A₂₆₀ = 0.84
A₂₈₀/A₂₆₀ = 0.98

dGTP (2´-Deoxyguanosine-5´-Triphosphate)
Formula (free acid): C₁₀H₁₆N₅O₁₃P₃

Molecular weight: 507
A₂₅₀/A₂₆₀ = 1.15
A₂₈₀/A₂₆₀ = 0.66

dTTP (2´-Deoxythymidine-5´-Triphosphate)
Formula (free acid): C₁₀H₁₇N₂O₁₄P₃

Formula weight: 482
A₂₅₀/A₂₆₀ = 0.65
A₂₈₀/A₂₆₀ = 0.71

dUTP (2´-Deoxyuridine-5´-Triphosphate)
Formula (free acid): C₉H₁₅N₂O₁₄P₃
Formula weight: 468

A₂₅₀/A₂₆₀ = 0.75
A₂₈₀/A₂₆₀ = 0.35

还可获得2＇-氟-2＇-脱氧胞苷-5＇-三磷酸（2＇-氟-dCTP）和2＇-氟-2＇-脱氧尿苷-5＇-三磷酸（2＇-氟-dUTP）的溶液。