3D细胞培养支架&3D水凝胶&3D Fect转染试剂解决方案

用于控制质粒DNA或siRNA递送的生物材料可以提供靶向转基因表达（过度表达或阻断）的基本工具，将为基因或细胞治疗提供新的视角。艾美捷 OZ Biosciences-3D细胞培养用转染试剂，针对基质胶和水凝胶优化！

3D基质类型：

艾美捷 3D细胞培养支架- 3D Scaffolds(#TF20250)

Collagen-based Scaffolds Collagen-derived Scaffolds Hyaluronic Acid MillicellTM (PTFE) Cell culture ｉnsert (Millipore) PCL，Polycaprolactone PEG，Poly(Ethylene Glycol) PLGA，Poly(lactic-co-glycolic acid) PS，Poly(Styrene) PU，Poly(Urethane)

3D水凝胶- 3D Hydrogels

Matrigel（BD）Collagen-Based Hydrogels Collagen-Derived Hydrogels Hyaluronic Acid Gelatin Extracellular Matrix Fibrin / Fibronectin Fibrinogen Laminin PEGylated hydrogels

发表文章：

细胞名称 核酸类型 产品 参考文章

CLU-301 3D Transfection DNA 3D-Fect Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

COS-1 3D Transfection DNA 3D-Fect Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

COS-7 3D Transfection DNA 3D-Fect Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HEK-293 3D Transfection DNA 3D-Fect Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HeLa 3D Transfection DNA 3D-Fect Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HMEC-1 3D Transfection DNA 3D-Fect Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

MDCK 3D Transfection DNA 3D-Fect Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

MEF 3D Transfection DNA 3D-Fect Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

Neural Stem Cells 3D Transfection DNA 3D-Fect Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

RWPE1 siRNA 3D-Fect Laperrousaz B., Nucleic Acids Res. 2018 Jan 31. doi: 10.1093/nar/gky030.

3T3 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

AdSC 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

BMSC 3D Transfection DNA 3D-FectIN Sukarto A., Eur Cells ａnd Mat. 2011; 22(4):28.

Chondrocytes 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

COS-1 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

COS-7 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HeLa 3D Transfection siRNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HMEC-1 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

Mesenchymal Stem Cells mRNA 3D-FectIN Ledo AM. Acta Biomater . 2020 May 15;S1742-7061(20)30224-5.

Mesenchymal Stem Cells mRNA 3D-FectIN Ledo AM., Biomaterials. 2020 Jul;247:120016.

Mesenchymal Stem Cells 3D Transfection DNA 3D-FectIN Plank C., Eur. J. Nanomed. 2012;4(1):17–32

Mesenchymal Stem Cells 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

Neural Stem Cells 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

NIH-3T3 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

RAW 264.7 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

RWPE1 siRNA 3D-FectIN Laperrousaz B., Nucleic Acids Res. 2018 Jan 31. doi: 10.1093/nar/gky030.

ATDC5 siRNA si3D-FectIN Mori H., Am J Pathol. 2015 Aug 10. pii: S0002-9440(15)00385-5.

Chondrocytes siRNA si3D-FectIN Mori H., Am J Pathol. 2015 Aug 10. pii: S0002-9440(15)00385-5.

3D-Fect Transfection Reagent

门开发用于直接转染在 3D 支架中培养的细胞。用3D-Fect / DNA复合物激活的基于支架的3D矩阵被细胞定植，随后在更自然的环境中原位转染。该方法可以研究组织工程和再生，肿瘤侵袭，神经分化，细胞化，组织形成，定植，神经突生长...   

结果展示：

3D-Fect 允许将多种细胞（细胞系和原代细胞）转染到 3D 支架中：基于胶原蛋白和胶原蛋白衍生的支架预加载由 3D-Fect（比例 4：1）和 1 μg pVectOZ-GFP（绿色图片）或 RFP 质粒（红色图片）形成的复合物。然后接种细胞并在48小时后在荧光显微镜下观察

来源：https://www.amyjet.com/featured/3d-fect-transfection.shtml