推荐：纤溶酶原 from human serum@2024已更新

别名:plasminogen

属性
生物来源
human serum

质量水平
100

形式
lyophilized

specific activity
~11 units/mg protein (activated with streptokinase at 37 °C ａnd pH 7.4 with Chromozym PL as the substrate.)

分子量
90-94 kDa

包装
pkg of 20 U

manufacturer/tradename
Roche

technique(s)
activity assay: suitable

application(s)
life science ａnd biopharma

运输
wet ice

说明
一般描述
Plasminogen is a profibrinolysin from human serum. Plasminogen, the proenzyme of the fibrinolytically active enzyme plasmin, is a single chain glycoprotein. Various isoelectric forms exist ａnd can be separated by means of isoelectric focusing (IEF). The lyophilizate is stabilized with bovine serum albumin (BSA).[1][2]
应用
Plasminogen is used for the determination of the activity of plasminogen activators.[3][4] The enzyme is a substrate for tissue plasminogen activator (t-PA) in the t-PA test ａnd a reference substance for t-PA therapy. It is used as the in-process control in the production of t-PA.
特点和优势
Contents
Lyophilizate, stabilized with bovine serum albumin.
质量
Contaminants: <0.05% plasmin; the preparation does not contain 6-aminohexanoic acid
序列
Native plasminogen (glu-plasminogen) has a N-terminal glutamic acid group. Partial plasma proteolysis gives rise to a molecule with N-terminal lysine (lys-plasminogen). Cleavage with elastase results in val-plasminogen. The complete amino acid sequence contains 790 amino acids. Roche Applied Science supplies glu-plasminogen.
制备说明
Stabilizers: stabilized with BSA
Working solution: Solvent is recommended in 20 mM sodium phosphate buffer, pH 7.5, 100 mM NaCl.
Storage conditions (working solution): -15 to -25 °C
A solution in 20 mM sodium phosphate buffer, pH ca. 7.5, 100 mM NaCl is stable for several months without loss of activity when stored at -15 to -25 °C.
Note:
Plasminogen is susceptible to autolysis, which may result in activation. Contact with the skin or other contamination (e.g., by microorganisms), is therefore to be avoided.
储存及稳定性
Store at 2 to 8 °C. (Store dry!)
其他说明
仅用于生命科学研究。不可用于诊断。