推荐：FRASER Listeria Ammonium iron (III) Supplement

别名:FRASER Listeria Ammonium iron(III) Supplement, Listeria Selective Supplement

经验公式（希尔记法）:C6H8O7 · xFe · xH3N

CAS号:1185-57-5

MDL编号:MFCD03099410

EC索引号:214-686-6

属性
Agency
ISO 11290
ISO

质量水平
200

形式
lyophilized powder

效能
>2000 mg/kg LD50, oral (Rat)

组成
Ammonium iron (III) citrate, 250 mg per vial

包装
pkg of 10 vials

technique(s)
microbiological culture: suitable

pH值（酸碱度）
6-8 (20 °C, 100 g/L in H2O)

溶解性
1200 g/L

application(s)
food ａnd beverages
microbiology
pathogen testing

相容性
GranuCult® FRASER Broth (Base) (1.10398)
GranuCult® Half FRASER (Demi FRASER) Broth (Base) (1.00025)

储存温度
15-25°C

适用性
Listeria spp.

InChI
1S/C6H8O7.Fe.H3N/c7-3(8)1-6(13,5(11)12)2-4(9)10;;/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);;1H3/q;+3;/p-3

InChI key
FRHBOQMZUOWXQL-UHFFFAOYSA-K

说明
一般描述
For preparation of FRASER broth, two different supplements are needed: FRASER Listeria Selective Supplement ａnd FRASER Listeria Ammonium Iron (III) Supplement. The ammonium iron (III) citrate promotes the growth of the Listeria spp., ａnd together with esculin (a component of the FRASER broth base), permits the β-D-glucosidase detection in Listeria spp.
应用
FRASER Listeria Ammonium iron (III) Supplement is an additive for the preparation of Half FRASER (Demi FRASER) Broth (Base) or FRASER Broth (Base) for the enrichment of Listeria spp.
联系
Replaces: 1000920017
分析说明
Growth promotion test according to ISO 11133
Mixed inoculum of Listeria, E.coli ａnd E.faecalis
Additionaly monoculture of E.coli ａnd E.faecalis
Pre-enrichment in Half Fraser Broth, afterwards enrichment in Fraser Broth
Confirmation on ALOA®-Medium resp. Tryptic soy agar
Inoculum on reference medium
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
10 - 100
- Listeria monocytogenes ATCC 13932 (WDCM 00021): 10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013): ≥ 1000
- Escherichia coli ATCC 8739 (WDCM 00012): ≥ 1000
- Enterococcus faecalis ATCC 19433 (WDCM 00009): ≥ 1000
- Enterococcus faecalis ATCC 29212 (WDCM 00087): ≥ 1000
Blacking
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
+
- Listeria monocytogenes ATCC 13932 (WDCM 00021): +
- Escherichia coli ATCC 25922 (WDCM 00013): -
- Escherichia coli ATCC 8739 (WDCM 00012): -
- Enterococcus faecalis ATCC 19433 (WDCM 00009): -
- Enterococcus faecalis ATCC 29212 (WDCM 00087): -
Growth on ALOA medium
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
>10 CFU
- Listeria monocytogenes ATCC 13932 (WDCM 00021): >10 CFU
Colonies
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
bluegreen with non transparent zone
- Listeria monocytogenes ATCC 13932 (WDCM 00021): bluegreen with non transparent zone
Growth on Tryptic soy agar
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): <100 CFU
- Enterococcus faecalis ATCC 19433 (WDCM 00009): <100 CFU
Incubation:
Half Fraser Broth: 24 hours at 30°C, aerobic
Fraser Broth: 48 hours at 37°C, aerobic
Confirmation on ALOA-Medium: 48 hours at 37°C, aerobic
Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic

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发表刊物        影响因子           奖励金额
SCI期刊杂志      1.0≤1F＜3.0          200元
SCI期刊杂志      3.0≤1F＜6.0          400元
SCI期刊杂志      6.0≤1F＜10.0         600元
SCI期刊杂志      1F≥10                1000元