【文献标题】Effect of erythropoietin combined with hypothermia on serum tau protein levels ａnd neurodevelopmental outcome in neonates with hypoxic-ischemic encephalopathy
【作者】Hong-yan Lv，Su-jing Wu，Qiu-li Wang，et.al
【作者单位】邯郸市邯郸妇幼保健院（Handan Maternal ａnd Child Health Care Hospital, Handan）
【文献中引用产品】
人Tau蛋白(TAU)ELISA试剂盒（http://www.ayijx.com/c149475/products/d4069323.html）
【关键词】nerve regeneration; erythropoietin; hypothermia; hypoxic-ischemic encephalopathy; neonate; tau protein; biomarkers; prognosis; neuroprotection; neural regeneration
【DOI】https://doi.org/10.4103/1673-5374.217338
【影响因子(IF)】3.6
【出版期刊】《NEURAL REGENERATION RESEARCH》
【产品原文引用】
Serum tau protein measurement
Blood samples were taken from the radial vein at 24 hours after birth (before EPO treatment) ａnd 4, 8, ａnd 12 days after EPO treatment. Blood samples were placed in a test tube (specifications: 5 mL, No A06277602; Hebi City Zhong Xing Medical Supplies, Hebi, China), maintained without stirring for 30 minutes at room temperature, ａnd then centrifuged in a L-400 low-speed automatic balancing centrifuge (Hunan Xiangyi Laboratory Instrument Development, Changsha, Hunan Province, China) at 3,000 r/min for 15 minutes.Serum was removed into another test tube ａnd placed in a freezer at ?70°C until use. To avoid any influence on the results, blood transfusions to the neonates were avoided before collecting blood samples. Serum tau protein was detected using an enzyme-linked immunosorbent assay. The kit was provided by the Shanghai HuDing Biological Science ａnd Technology Co., Ltd.