【文献标题】Anti-inflammation Effects of Cordyceps sinensis Mycelium in Focal Cerebral Ischemic Injury Rats
【作者】Zhenquan Liu,Pengtao Li,Dan Zhao,et.al
【作者单位】北京中医药大学（ Beijing University of Chinese Medicine）
【文献中引用产品】大鼠白介素1β (IL-1β)ELISA试剂盒（http://www.ayijx.com/c153249/products/d4338172.html）
大鼠肿瘤坏死因子α（TNF-α）ELISA试剂盒（http://www.ayijx.com/c153249/products/d4338168.html）【关键词】CSM; inflammation; ischemia–reperfusion; inflammatory cells; inflammatory mediators.
【DOI】https://10.1007/s10753-010-9273-5
【影响因子(IF)】5.5
【出版期刊】《Inflammation》
【产品原文引用】
Measurement of IL-1β ａnd TNF-α Level
Protein concentration of interleukin-1β (IL-1β) ａnd tumor necrosis factor-α (TNF-α) were measured by the way introduced by Z. Cai [20]. Briefly, rats were killed after grip neurobehavioral test. Protein was extracted from the whole brain ａnd the concentration was adjusted to 4 mg/ml. The brain concentration of
IL-1β,
TNF-α was then determined using a commercial enzyme-linked immunosorbent assay (ELISA) kit (
Shanghai Jinma Biological Technology, Inc., China) following the manufacture’s instruction.
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