【文献标题】GLP-1 receptor regulates cell growth through regulating IDE expression level in
Aβ1-42-treated PC12 cells
【作者】Huajie Li,Liping Cao,Yi Ren,et.al
【作者单位】常州第一人民医院神经内科（Department of Neurology, the First People’s Hospital of Chang Zhou, Jiang Su）
【文献中引用产品】杆菌肽(Bacitracin)ELISA试剂盒
【关键词】GLP-1 receptor, IDE expression, Alzheimer＇s disease, type 2 diabetes
【DOI】http://dx.doi.org/10.1042/BSR20171284
【影响因子(IF)】2.9
【出版期刊】《Bioscience Reports》
【产品原文引用】Cell culture ａnd treatments
PC12 cells were obtained from Shanghai Institute for Cell Research, Chinese Academy of Sciences. The cells were grown in DMEM supplemented with 10% foetal bovine serum ａnd 1% antibiotics(penicillin/streptomycin, Sigma-Aldrich, St Louis, MO, USA) in a humidified atmosphere of 95% air ａnd 5% CO2 at 37°C. The medium was changed every 2-3 days. Aβ1-42 oligomers were obtained from Sigma (Sigma-Aldrich, St Louis, MO, USA) ａnd were added to cell cultures at a final concentration of 0, 1.25, 2.5, 5 ａnd 10 μM. Gen was obtained from the National Institute for the Control of Pharmaceutical ａnd Biological Products ａnd was added to cell cultures at a final concentration of 0, 0.1, 1.0 ａnd 10 μM. For treatment with IDE antagonist
bacitracin (Bac), PC12 cells were incubated with 1 mg/L Bac (
Shanghai Huding technology Co., Ltd., China) for 30 min. For PKA inhibitor experiment, PC12 cells were treated with 20 μM H89 (Calbiochem, La Jolla, CA), which was prepared as a 4 mM stock solution in DMSO.
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