推荐：GranuCult® Mannitol egg yolk polymyxin agar (base)

别名:MYP agar, Bacillus Cereus Selective Agar, Cereus Selective Agar Base, MYEP agar

属性
Agency
APHA
ISO 21871
ISO 7932

质量水平
200

reg. compliance
FDA (BAM)

形式
medium granules (dehydrated (DCM))

manufacturer/tradename
GranuCult®

technique(s)
microbiological culture: suitable

pH值（酸碱度）
7.0-7.4 (25 °C, 43 g/L in H2O, after autoclaving)

溶解性
43 g/L

application(s)
food ａnd beverages
microbiology

储存温度
15-25°C

适用性
Bacillus cereus

说明
一般描述
GranuCult® Mannitol egg yolk polymyxin agar (base) is used for the enumeration of Bacillus cereus. This medium contains two indicator systems, mannitol, ａnd phenol red ａnd egg yolk. Enzymatic digest of casein ａnd meat extract provide carbon ａnd nitrogen sources. The selective inhibitory component polymyxin suppresses the growth of many unwanted microorganisms. Bacteria that ferment mannitol to produce acids produce yellow staining of the medium with phenol red as a pH indicator. Bacillus cereus does not ferment mannitol so the medium around the colonies remains unchanged or is discolored due to light alkalization by forming pink colonies. The lecithin present in egg yolk is cleaved by the Bacillus cereus-lecithinase, which leads to the formation of an opalescent white precipitation zone surrounding the pink colonies. Bacillus anthracis, Bacillus mycoides, Bacillus pseudodomycoides, Bacillus thuringiensis, ａnd Bacillus weihenstephanensis may not be distinguishable from Bacillus cereus on this medium.
应用
GranuCult® Mannitol egg yolk polymyxin agar (base) is used for the enumeration of presumptive Bacillus cereus from food ａnd animal feed as well as from environmental samples ａnd other materials.
分析说明
Appearance (clearness): clear to slightly opalescent
Appearance (color): red
pH-value (25 °C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Bacillus cereus ATCC 11778 (WDCM 00001)):
Colony ｃount (Bacillus cereus ATCC 11778 (WDCM 00001)):
Recovery on test medium (Bacillus cereus ATCC 11778 (WDCM 00001)): ≥ 50 %
Growth (Bacillus subtilis ATCC 6633 (WDCM 00003)): no limit
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Colonies (Bacillus cereus ATCC 11778 (WDCM 00001)): pink colonies with precipitation halo
Colonies (Bacillus subtilis ATCC 6633 (WDCM 00003)): yellow colonies without precipitation halo
Incubation:
B.cereus 24 ± 3 to 44 ± 4 hours at 30 ± 1 °C
other teststrains 44 ± 4 hours at 30 ± 1 °C
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar
附注
We offer two media types: the superior granulated GranuCult® ａnd the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

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发表刊物        影响因子           奖励金额
SCI期刊杂志      1.0≤1F＜3.0          200元
SCI期刊杂志      3.0≤1F＜6.0          400元
SCI期刊杂志      6.0≤1F＜10.0         600元
SCI期刊杂志      1F≥10                1000元