推荐：HiCrome™ ECC 选择性培养基@2024已更新

别名:ECC 选择性培养基 HiCrome™, 大肠杆菌-大肠菌选择性培养基 HiCrome™

MDL编号:MFCD01634645

NACRES:NA.85

属性
无菌性
non-sterile

质量水平
200

形式
powder

保质期
limited shelf life, expiry date on the label

组成
agar, 10.0 g/L
casein enzymic hydrolysate, 3.3 g/L
chromogenic mixture, 0.43 g/L
disodium hydrogen phosphate, 1 g/L
peptone, special, 6.0 g/L
sodium chloride, 2 g/L
sodium dihydrogen phosphate, 0.6 g/L
sodium pyruvate, 1 g/L
sorbitol, 1 g/L
Tergitol™
7, 0.15 g/L
tryphan, 1 g/L

manufacturer/tradename
NutriSelect® Plus

终pH
6.8±0.2 (25 °C)

application(s)
agriculture
bioburden testing
environmental
food ａnd beverages
water monitoring
microbiology

适用性
selective ａnd differential for Citrobacter spp.
selective ａnd differential for Enterobacter spp.
selective ａnd differential for Escherichia coli
selective ａnd differential for Salmonella spp.
selective ａnd differential for Shigella spp.
selective ａnd differential for coliforms
selective ａnd differential for enterobacteriaceae

说明
应用
HiCrome™ ECC 选择性琼脂是一种选择性培养基，推荐用于对水和食物样品中的大肠杆菌和大肠菌群进行同时检测。该培养基中的成分甚至有助于亚致死性损伤的大肠菌迅速生长，Tergitol™ 可以抑制革兰氏阳性和某些革兰氏阴性细菌，而不影响大肠菌。显色混合物包含两种显色底物：Salmon-GAL 和 X-葡萄糖苷酸。大肠菌产生的 β-D-半乳糖苷酶可裂解 Salmon-GAL，使大肠菌菌落呈现鲜肉色至红色。大肠杆菌产生的 β-D-葡萄糖醛酸苷酶则可裂解 X-葡萄糖苷酸。由于 Salmon-GAL 和 X-葡萄糖苷酸均被裂解，因此大肠杆菌形成深蓝色到紫色的菌落。添加色氨酸可增进吲哚反应。为了确认大肠杆菌，可将一滴柯氏试剂（目录号 60983）滴在深蓝色到紫色的菌落上。形成樱桃红色则说明反应呈阳性。
制备说明
Suspend 26.5 g in 1 litre distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas ａnd Aeromonas species add 5 mg Cefsulodin (Cat. No. 22126) for surface ａnd pour plate method ａnd 10 mg Cefsulodin for membrane filter technique. Mix well.
重悬
Suspend 26.5 g in 1 liter of distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas ａnd Aeromonas species, add 5 mg of cefsulodin for surface ａnd pour plate method ａnd 10 mg of cefsulodin for membrane filter technique. Mix well.
附注
We offer two media types: the superior granulated GranuCult® ａnd the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

IF<5，赠送价值 500元礼品卡
5 ≤IF≤10，赠送价值 1000元礼品卡
IF≥10，赠送价值 1000元礼品卡+任意 1 款 ValukineTM 试剂盒


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发表刊物        影响因子           奖励金额
SCI期刊杂志      1.0≤1F＜3.0          200元
SCI期刊杂志      3.0≤1F＜6.0          400元
SCI期刊杂志      6.0≤1F＜10.0         600元
SCI期刊杂志      1F≥10                1000元